广西植物2025，Vol.45Issue(12)：2184-2199,16.DOI:10.11931/guihaia.gxzw202404053
高海拔地区油茶叶片解剖结构与抗旱性关系研究
Leaf anatomical structure of Camellia oleifera and its relationship with drought resistance in high altitude areas
摘要
Abstract
In order to explore the anatomical structure characteristics of Camellia oleifera leaves in high altitude areas and the relationship between their structure and drought resistance,In this study,35 superior individual plants of C.oleifera with excellent fruiting traits,previously selected in the high altitude areas of eastern Guizhou Province,were used as experimental materials,and observed the leaf anatomical structure using paraffin sections.Typical indicators of leaf structure that reflected plant drought resistance were screened out through descriptive and variance analysis,correlation analysis and cluster analysis,and then the membership function was used to comprehensively evaluate drought resistance and select superior individual plants with strong drought resistance.The results were as follows:(1)The number of cell layers in C.oleifera palisade tissue varied.Most were composed of two layers of neatly arranged and dense long columnar cells,and a few had three layers.The coefficient of variation of morphological indicators ranged from 11.15%to 26.73%,and the coefficient of variation of the ratio of palisade tissue to spongy tissue was the largest among the 14 indicators.(2)Through cluster analysis and comprehensive ranking of related indexes,it was concluded that the main indicators affecting the drought resistance of C.oleifera were ratio of palisade tissue to spongy tissue,leaf area,vein thickness and palisade tissue thickness.TY05 had a maximum vein thickness of 599.32 μm,and TY16 had a minimum vein thickness of 347.53 μm.The largest leaf area was TY33,which was 1766.00 mm2 and had two layers of palisade tissue cells.TY08 had the maximum leaf thickness,palisade tissue thickness and ratio of palisade tissue to spongy tissue,which were 673.33 μm,340.26 μm and 1.13,respectively.(3)According to the membership function comprehensive value,TY26,TY08,TY03,TY27,TY33 had strong drought resistance,which could provide a material basis for the next step of drought resistance variety breeding.The research results provide a scientific basis and theoretical reference for the breeding of drought resistant varieties of C.oleifera in high altitude areas.关键词
高海拔/油茶/叶片/解剖结构/抗旱性Key words
high altitude/Camellia oleifera/leaf/anatomical structure/drought resistance分类
农业科技引用本文复制引用
孙冬婵,高超,宋启玲,万先琴,聂艳美,王启梅..高海拔地区油茶叶片解剖结构与抗旱性关系研究[J].广西植物,2025,45(12):2184-2199,16.基金项目
国家自然科学基金(32360404,32060331) （32360404,32060331）
贵州省科技计划项目(黔科合支撑[2022]重点 017 号) （黔科合支撑[2022]重点 017 号）
黔东南州科技计划项目(黔东南科合支撑[2023]03 号). （黔东南科合支撑[2023]03 号）
