广西植物2025，Vol.45Issue(12)：2200-2213,14.DOI:10.11931/guihaia.gxzw202410017
基于MSAP技术对不同蒙古黄芪居群表观遗传多样性的研究
Epigenetic diversity of different populations of Astragalus membranaceus var.mongholicus based on MSAP technique
摘要
Abstract
To explore the epigenetic characteristics of different populations of Astragalus membranaceus var.mongholicus,a total of 259 A.membranaceus var.mongholicus samples from eight populations,including Wuchuan in Inner Mongolia,Zizhou in Shaanxi,Hunyuan in Shanxi etc.,were analyzed for DNA methylation levels and patterns using methylation sensitive amplified polymorphism(MSAP)technology,combined with population epigenetic diversity,population epigenetic structure,and NJ(neighbor-joining)cluster analysis.The results were as follows:(1)Ten pairs of selective amplification primers were screened for good polymorphisms,with the percentage of methylation-sensitive polymorphic loci ranging from 50%to 79%.(2)The average level of total methylation(62.64%)in A.membranaceus var.mongholicus was greater than that of unmethylation 37.36%,the average level of full methylation(33.39%)was greater than that of hemi-methylation(29.25%),and the full methylation pattern was predominant.Simultaneously,the average level of total methylation was lower in the wild population(58.97%)than in the cultivated population(64.84%).(3)The level of epigenetic diversity(I:0.555 5±0.121 0)was higher than that of genetic diversity(I:0.209 7±0.102 1)in A.membranaceus var.mongholicus populations,where the level of epigenetic diversity in the wild population(0.5531±0.1148)was slightly lower than that of the cultivated population(0.5569±0.1247),and the level of genetic diversity(0.211 7±0.054 0)was higher than that of the cultivated population(0.208 5±0.113 9);AMOVA analysis showed that epigenetic variation mainly occurred within populations(79%).(4)Both the epigenetic structure of the populations and NJ cluster analysis divided the eight populations of A.membranaceus var.mongholicus into four groups,and the populations were preferentially clustered by geography,showing some epigenetic similarity.The research results on DNA methylation,population epigenetic diversity,population epigenetic structure,and NJ cluster analysis of A.membranaceus var.mongholicus collectively reveal the epigenetic characteristics of A.membranaceus var.mongholicus population,providing a new idea for further understanding the phenotypic variation and adaptive strategies of A.membranaceus var.mongholicus under different environmental conditions.Simultaneously,this study provides an epigenetic perspective for formulating a reasonable resource protection strategy for A.membranaceus var.mongholicus and conducting the breeding of excellent traits.关键词
蒙古黄芪/DNA甲基化/甲基化敏感扩增多态性/表观遗传多样性/表观遗传结构Key words
Astragalus membranaceus var.mongholicus/DNA methylation/methylation sensitive amplified polymorphism/epigenetic diversity/epigenetic structure分类
农业科技引用本文复制引用
梁云慧,高慧霞,姚妙卓,刘澄玉,张鹏飞,刘亚令..基于MSAP技术对不同蒙古黄芪居群表观遗传多样性的研究[J].广西植物,2025,45(12):2200-2213,14.基金项目
国家自然科学基金(32070378) （32070378）
山西省现代农业产业技术体系建设专项资金(2021-11) （2021-11）
山西农业大学生物育种工程项目(YZGC136). （YZGC136）
