广西植物2025，Vol.45Issue(12)：2214-2226,13.DOI:10.11931/guihaia.gxzw202409047
甘草的遗传多样性和谱系地理结构的研究
Genetic diversity and phylogeographic structure of Glycyrrhiza uralensis
摘要
Abstract
To reveal the genetic diversity and phylogeographic structure of Glycyrrhiza uralensis,this study amplified and sequenced 100 samples from 20 populations of G.uralensis based on ITS and three chloroplast gene sequences(mat K,psb A-trnH,and trnS-trnG),and analyzed the data.The results were as follows:(1)The length of ITS sequence was 691 bp,containing 63 variable sites,with a haplotype diversity of 0.265 and a nucleotide diversity of 0.002 69;the length of cpDNA sequence was 1 976 bp,containing 740 variable sites,with a haplotype diversity of 0.907 and a nucleotide diversity of 0.026 39.These findings demonstrated high genetic diversity at the species level.(2)Molecular variance analysis result revealed that the genetic differentiation of G.uralensis primarily occurred within populations,with relatively low levels of genetic differentiation between populations and relatively high gene flow(ITS:Nm=1.349;cpDNA:Nm=1.520).(3)The comparison results of genetic differentiation coefficients(Nst and Gst)indicated that there was no significant phylogeographic structure within G.uralensis populations.The results of neutral test and mismatch analysis curve suggested that the overall G.uralensis population did not experience expansion events.(4)Haplotype geographical distribution analysis results revealed that G.uralensis in Shaanxi,Qinghai,Xinjiang,Inner Mongolia and Gansu exhibited relatively high haplotype diversity and was located at the center of the geographical distribution map,suggesting that this region might have served as a glacial refuge of G.uralensis in Northwest and North China.The study analyzed the genetic diversity and geographical distribution patterns of G.uralensis using different DNA barcodes,providing a theoretical basis for the conservation and rational utilization of G.uralensis germplasm resources in the future.关键词
甘草/ITS序列/cpDNA序列/地理分布格局/谱系地理学Key words
Glycyrrhiza uralensis/ITS sequence/cpDNA sequence/geographical distribution pattern/phylogeography分类
农业科技引用本文复制引用
高慧霞,梁云慧,姚妙卓,刘澄玉,张鹏飞,刘亚令..甘草的遗传多样性和谱系地理结构的研究[J].广西植物,2025,45(12):2214-2226,13.基金项目
山西省回国留学人员科研资助项目(2020-069) （2020-069）
国家自然科学基金(32070378) （32070378）
山西省现代农业产业技术体系建设专项资金(2021-11) （2021-11）
山西农业大学生物育种工程项目(YZGC136). （YZGC136）
