广西植物2025，Vol.45Issue(12)：2227-2240,14.DOI:10.11931/guihaia.gxzw202412031
工业大麻WOX基因家族的全基因组鉴定及表达分析
Genome-wide identification and expression analysis of WOX gene family in industrial hemp
摘要
Abstract
Industrial hemp is an important fiber crop,and its fibers have multiple unique advantages and broad application prospects.WOX gene family has potential roles in regulating fiber development,plant growth,and stress resistance in industrial hemp.Currently,the functions of the CsWOX gene family in industrial hemp and its roles in key biological processes such as stable yield and yield increase have not been clarified.Therefore,this study used bioinformatics methods to analyze the physicochemical properties,conserved domains,and expression patterns of the CsWOX genes in industrial hemp,to reveal their molecular functions in fiber development and drought stress response,and to preliminarily clarify the molecular mechanism by which Trichoderma hamatum enhances plant stress resistance through regulating the WOX gene network.The results were as follows:(1)Eleven CsWOX gene family members were identified in industrial hemp,encoding proteins with number of amino acids ranging from 223 to 435 aa,and relative molecular weights ranging from 25 398.30 to 48429.45 Da.Most proteins in this gene family were acidic and localized in the nucleus.(2)CsWOX proteins could be divided into three branches(ancient branch,intermediate branch,and modern branch).The modern branch contained six sub-branches,the intermediate branch contained two sub-branches,and the ancient branch contained only one sub-branch.WOX transcription factors in the same sub-branch had certain conservation in the types and organization of conserved motifs.Each family member contained a conserved domain with a helix-loop-helix-turn-helix structure.(3)Promoter prediction analysis found that the number of cis-elements related to light response,gibberellin response,and abscisic acid response was the highest.Other cis-elements such as auxin response,salicylic acid response,anaerobic response,circadian rhythm response,low temperature response,and defense response were unevenly distributed in the promoters.(4)CsWOX4 and CsWOX13a/CsWOX13b genes showed high expression levels in roots,stems,leaves,and flowers.Compared with the control group,the expression levels of CsWOX4 and CsWOX13a/CsWOX13b changed under drought treatment,Trichoderma hamatum treatment,and combined drought and Trichoderma hamatum treatment,suggesting that they may play potential roles in the growth,development,and stress resistance of industrial hemp.This study reveals the molecular functions of CsWOX genes in growth,development,and drought stress response,preliminarily clarifies the molecular mechanism by which Trichoderma hamatum enhances plant stress resistance through regulating the WOX gene network,and provides important clues for understanding the functions of the CsWOX gene family in the growth,development,and stress resistance of industrial hemp.关键词
工业大麻/WOX基因家族/全基因组鉴定/生物信息学分析/分子育种Key words
industrial hemp(Cannabis sativa)/WOX gene family/genome-wide identification/bioinformatics analysis/molecular breeding分类
农业科技引用本文复制引用
田岳,黄紫樱,刘圆圆..工业大麻WOX基因家族的全基因组鉴定及表达分析[J].广西植物,2025,45(12):2227-2240,14.基金项目
国家自然科学基金(32200226) （32200226）
福建农林大学海峡联合研究院麻类作物基因改良项目(125-KH190236A). （125-KH190236A）
