广西植物2025，Vol.45Issue(12)：2255-2270,16.DOI:10.11931/guihaia.gxzw202409026
棱枝槲寄生叶绿体基因组结构特征及系统发育分析
Chloroplast genome structure characteristics and phylogenetic analysis of Viscum diospyrosicola
摘要
Abstract
To elucidate the characteristics of chloroplast genome of Viscum diospyrosicola and resolve the phylogenetic relationships within the Viscum genus.Utilizing the Illumina high-throughput sequencing platform,we sequenced its chloroplast genome and performed bioinformatics analyses,including assembly,annotation,and comparative genomic analysis.The results were as follows:(1)The chloroplast genome of V.diospyrosicola exhibited a typical circular quadripartite structure,with the total length of 126315 bp and the total GC content of 36.40%.A total of 111 genes were annotated,including 69 protein-coding genes,34 tRNA genes,and 8 rRNA genes,with no detection of the transcription initiation factor infA.(2)Comparative genomic analysis revealed that the variation rate in the LSC region of the chloroplast genome was significantly higher than that in the IR region among five Viscum species.Specific variations were found in the coding regions of genes such as ycf1,ycf2,ycf3,atpA,rpoC2,and rpoC1,providing potential targets for molecular marker development.(3)Codon usage bias analysis showed that the third base of codons predominantly ended with A/U,and the codon usage pattern was mainly influenced by natural selection.(4)Phylogenetic analysis results indicated that the species were divided into two main branches,Loranthaceae and Santalaceae,with V.diospyrosicola clustering with V.liquidambaricola,indicating the closest genetic relationship.This study is the first to elucidate the characteristics of the chloroplast genome of V.diospyrosicola and to clarify the phylogenetic relationships within the Viscum,providing a theoretical basis for molecular identification and phylogenetic research of Viscum species.关键词
棱枝槲寄生/槲寄生属/叶绿体基因组/序列比对/密码子偏好性/系统发育分析Key words
Viscum diospyrosicola/Viscum/chloroplast genome/sequence alignment/codon usage bias/phylogenetic analysis分类
农业科技引用本文复制引用
张煜冰,曾晓丽,班启明,郑红军,邱建生..棱枝槲寄生叶绿体基因组结构特征及系统发育分析[J].广西植物,2025,45(12):2255-2270,16.基金项目
贵州省科技计划项目(黔科合服企[2020]4010). （黔科合服企[2020]4010）
