广西植物2025，Vol.45Issue(12)：2284-2295,12.DOI:10.11931/guihaia.gxzw202408041
低温胁迫下兴安白芷与川白芷种子转录组特征及激素调控基因的比对研究
Comparative study on the transcriptome characteristics and hormone regulatory genes of Angelica dahurica and Angelica dahurica cv.'Hangbaizhi'seeds under low-temperature stress
摘要
Abstract
To analyze the effects of low-temperature stress on seed germination of Angelica dahurica and A.dahurica cv.'Hangbaizhi',the cold resistance of seeds from different A.dahurica cultivars and the key genes were explored.Based on the comparative experiment of low-temperature and normal-temperature germination of A.dahurica and A.dahurica cv.'Hangbaizhi'seeds,transcriptome sequencing was performed in different treatment groups to analyze the differential expressions of key hormones abscisic acid(ABA)and gibberellins(GA)during seed germination under low-temperature stress.The outcomes were verified by qRT-PCR.The results were as follows:(1)The seed vigor of the two A.dahurica varieties significantly decreased under low-temperature conditions,and the seed vigor of A.dahurica was significantly higher than that of A.dahurica cv.'Hangbaizhi'.(2)Transcriptome analysis showed that the expression levels of Cu/ZnSOD,CYP707A,and CAT significantly decreased under low-temperature conditions;while the expressions of ABA(PYL)and GA(SNE,GIS)-related genes significantly increased.(3)In the two varieties,A.dahurica had higher expression levels of PsaA and BIP,while A.dahurica cv.'Hangbaizhi'had higher expression of ABI.(4)The decrease in seed activity of two varieties of A.dahurica under low-temperature stress was mainly related to the significant reduction in the expressions of key genes such as Cu/ZnSOD,CYP707A,and CAT;the differences in seed germination between the two A.dahurica varieties were mainly related to the differential expressions of ABA and GA related genes.In conclusion,the synergistic work of these factors ultimately significantly affects seed activity.This study provides new genetic references for the research of cold resistance in seeds of A.dahurica and A.dahurica cv.'Hangbaizhi'.关键词
兴安白芷/川白芷/低温胁迫/植物激素信号转导/种子萌发/转录组Key words
Angelica dahurica/Angelica dahurica cv.'Hangbaizhi'/low temperature stress/plant hormone signal transduction/seed germination/transcriptome分类
农业科技引用本文复制引用
刘依婷,骆冰倩,王雅兰,李永宁,高继海..低温胁迫下兴安白芷与川白芷种子转录组特征及激素调控基因的比对研究[J].广西植物,2025,45(12):2284-2295,12.基金项目
中央财政重点项目国家中医药跨学科创新团队(ZYYCXTD-D-202209) （ZYYCXTD-D-202209）
成都中医药大学杏林学者项目(QJRC2022029) （QJRC2022029）
特色中药资源多维评价与产品开发创新团队项目(2022C001) （2022C001）
川产道地药材大品种精深加工关键技术研究与产品开发示范项目(2020YFN0152). （2020YFN0152）
