广西植物2025，Vol.45Issue(12)：2308-2321,14.DOI:10.11931/guihaia.gxzw202412009
20种胡椒属植物叶表皮微形态特征及其分类学意义
Micromorphological characteristics of leaf epidermis and taxonomic significance of 20 species of Piper L
摘要
Abstract
The genus Piper is characterized by a notable degree of interspecific morphological similarity coupled with considerable intraspecific variation,which presents significant challenges for accurate species classification.To address taxonomic issues and explore the taxonomic significance of leaf epidermal micromorphological characteristics in Piper species from China,this study systematically examined and compared the characteristics of Piper nigrum and 19 native Piper species using light microscopy(LM)and scanning electron microscopy(SEM).The results were as follows:(1)The leaf epidermal cells of most species were polygonal in shape,exhibiting straight-arched anticlinal walls.In contrast,only a few species displayed irregularly shaped epidermal cells with sinuolate anticlinal walls on the upper epidermis.(2)Stomatal apparatus were exclusively located on the lower epidermis and could be classified into three distinct types:anisocytic,amphicyclocytic,and the transitional type between these two categories.Size and density of stomatal apparatus showed significant interspecific variation,serving as auxiliary diagnostic features for species differentiation.(3)The cuticular ornamentation was categorized into four main types:nearly smooth,striate,papillate,and micropapillate.These distinct patterns of ornamentation partially reflect the phylogenetic relationships among species and provide valuable insights for the infrageneric classification within the genus Piper.(4)Trichomes were classified into two broad categories:glandular and non-glandular types.The majority of species exhibited glandular trichomes,while non-glandular trichomes included both simple and branched types.A small number of species were glabrous.The characteristics of trichomes were demonstrated to be critical and informative taxonomic criterion for interspecific classification within Piper.(5)Based on the leaf epidermal micromorphological characteristics and other relevant traits,the taxonomic status of certain species was re-evaluated.This study supported the recognition of P.thomsonii var.microphyllum as a distinct species and rejected the synonymization of P.curtipedunculum with P.pedicellatum.Additionally,it affirmed P.semi-transparens as a separate species,while also confirming the close phylogenetic relationship between P.flaviflorum and P.nigrum.The results of this study indicate the leaf epidermal micromorphological characteristics provide an important basis for the classification of Piper.关键词
叶表皮/胡椒属/微形态/扫描电镜/分类Key words
leaf epidermis/Piper L./micromorphology/scanning electron microscope/taxonomy分类
农业科技引用本文复制引用
冯元元,范睿,刘正铸,苏凡,吉训志,郝朝运..20种胡椒属植物叶表皮微形态特征及其分类学意义[J].广西植物,2025,45(12):2308-2321,14.基金项目
海南省现代农业产业技术体系专项(HNARS-09-G01) （HNARS-09-G01）
科技部、财政部国家科技资源共享服务平台(NTPGRC2025-014) （NTPGRC2025-014）
国家现代农业产业技术体系项目(CARS-11). （CARS-11）
