核农学报2026，Vol.40Issue(2)：268-278,11.DOI:10.11869/j.issn.1000-8551.2026.02.0268
盐地碱蓬内生耐盐碱枯草芽孢杆菌NXO3的促生抑菌潜力初探
Preliminary Study on the Growth-Promoting and Antifungal Potential of Saline-Alkali Tolerant Endophytic Bacillus subtilis Isolated from Suaeda salsa
摘要
Abstract
To obtain saline-alkali tolerant bacterial strains with plant growth-promoting properties,an endophytic strain NXO3 was isolated from the stems and leaves of Suaeda salsa through selective culture screening in this study.The bacterial strain showed phosphorus solubilization,nitrogen fixation,indole acetic acid(IAA)production,and polyglutamic acid synthesis,as well as significant antagonistic activity against various plant pathogens.The strain was further identified as Bacillus subtilis NXO3 using morphological observation,physiological and biochemical characteristics analysis,and molecular biology methods.Whole-genome sequencing was performed to predict and analyse the NXO3 genes and gene clusters involved in the biosynthesis of stress-resistant plant growth-promoting metabolites like tryptophan,IAA,and glycine betaine.The analysis identified genes related to salt tolerance,including those encoding Na+/H+antiporter and K+-Cl-cotransport proteins.Under 0.24％sodium bicarbonate alkaline stress conditions,treatment with 20％fermentation broth of the strain NXO3 for 72 h significantly enhanced the germination rate of wheat seeds to 86.67％and cucumber seeds to 85.00％,with both outcomes showing significant improvements compared to the control groups(P＜0.05).Furthermore,stain NXO3 was formulated into an agricultural microbial inoculant,when applied to mildly saline-alkali coastal sandy soil(salt content 0.1％-0.3％)at 750 kg·hm-2,the inoculant significantly improved peanut germination rate to 83.15％and increased yield to 2 381.40 kg·hm-2 compared to the control group(P＜0.05).While in light to moderate saline-alkali soil(salinity content 0.1％-0.5％),application of 1 200 kg·hm-2 of NXO3 resulted in a yield of 5 785.65 kg·hm-2,showing significant difference from the control group(P＜0.05).The field experiments showed this agricultural microbial inoculant can improve the emergence rate and yield of peanuts in saline-alkali soil.This study provides a scientific foundation for developping agricultural microbial agents based on B.subtilis stain NXO3 for saline-alkali soil remediation,thereby enhancing agricultural productivity and promoting ecological sustainability in such soils.关键词
盐碱地/盐地碱蓬/枯草芽孢杆菌/花生/农用微生物菌剂Key words
saline-alkali soil/Suaeda salsa/Bacillus subtilis/peanut/agricultural microbial inoculant引用本文复制引用
张佳蕊,王晓晗,李东海,张梓妍,李陆杨,谭鑫淼,韩会玲,孙伟明..盐地碱蓬内生耐盐碱枯草芽孢杆菌NXO3的促生抑菌潜力初探[J].核农学报,2026,40(2):268-278,11.基金项目
河北省现代农业产业技术体系建设专项资金(HBCT2024040203),河北省重点研发计划项目(花生现代种业科技创新团队21326316D-6),河北科技师范学院博士启动基金项目(2023YB015) （HBCT2024040203）
