海洋科学2025，Vol.49Issue(6)：98-111,14.DOI:10.11759/hykx20250224001
栉孔扇贝内脏多糖的提取分离与生物活性研究
Extraction,isolation,and bioactivity of visceral polysaccha-rides from Chlamys farreri
摘要
Abstract
In this study,we examined the extraction,separation,and activity of polysaccharides from Chlamys far-reri viscera byproducts.The crude polysaccharides were primarily prepared using the enzyme digestion method.The optimal enzyme digestion conditions were identified using a one-way experiment,which revealed an optimal neutral protease to trypsin ratio of 1∶2 at pH 7,a reaction temperature of 40℃,and a digestion time of 2 h.The crude polysaccharides were subjected to separation and purification using a DEAE-52 ion exchange column and a Sephadex G-100 gel exclusion chromatographic column,respectively.The molecular weight of the purified CFP4-G3 fraction was 4 935 Da,with a uronic acid content of 4.081%and a sulfate content of 4.335%.Infrared absorption spectroscopy and ultraviolet spectroscopy confirmed that CFP4-G3 conformed to the typical structural features of amino polysaccharides.Bioactivity assays of the resulting CFP4-G3 polysaccharide demonstrated that it had strong antioxidant activity,including scavenging of hydroxyl radicals,superoxide anion,and DPPH,and also possessed substantial reducing power.In addition,CFP4-G3 exhibited strong immunostimulatory activity.Moreover,CFP4-G3 substantially stimulated NO release and the secretion of TNF-α and IL-6 in RAW264.7 mouse macro-phages.The above findings provide a crucial theoretical foundation for the in-depth development of scallop viscera polysaccharides.关键词
栉孔扇贝/内脏/多糖/分离纯化/生物活性Key words
Chlamys Farreri/visceral/polysaccharides/isolation and purification/bioactivities分类
生物科学引用本文复制引用
王昕,刘畅,葛香云,邢荣娥,刘松,李克成..栉孔扇贝内脏多糖的提取分离与生物活性研究[J].海洋科学,2025,49(6):98-111,14.基金项目
国家重点研发计划专项课题(2023YFD2401105) （2023YFD2401105）
现代农业产业技术体系专项(CARS-49)the National Key R&D Program of China,No.2023YFD2401105 （CARS-49）
the Earmarked Fund for Modern Agro-industry Technology Research System,No.CARS-49 （）
