microRNA-183-5p通过调控MITF基因影响长牡蛎黑色素形成
Regulation of melanin formation in Crassostrea gigas by mi-croRNA-183-5p via MITF gene
摘要
Abstract
Melanin synthesis is an extremely complex process involving multiple genes and microRNAs(miRNAs).In recent years,numerous studies have confirmed that miRNAs play important roles in mammalian coat color for-mation.However,only a few studies have examined the mechanisms by which miRNAs regulate melanin synthesis in shellfish.In Crassostrea gigas(C.gigas),the mantle is closely related to melaninosis,a key component of shell color formation and melanin production.In this study,RNAhybrid was used to predict the target genes of miR-183-5p.The effects of miR-183-5p on melanin synthesis were verified by quantitative real-time polymerase chain reaction(qRT-PCR),dual-luciferase reporter gene assays,and Masson-Fontana melanin staining.In silico predictions and dual-luciferase reporter gene assays confirmed that microphthalmia-associated transcription factor(MITF)is a target of miR-183-5p.qRT-PCR analysis revealed that miR-183-5p is expressed at higher levels in white than in black mantles of C.gigas.The overexpression of miR-183-5p significantly downregulated genes related to melanin formation.Melanin staining showed that the brown particles in the mimic group were markedly reduced compared to those in the negative control group.In conclusion,miR-183-5p may regulate melanin formation in C.gigas by targeting MITF.关键词
长牡蛎(Crassostrea gigas)/黑色素/miR-183-5p/MITFKey words
Crassostrea gigas/melanin/miR-183-5p/MITF分类
农业科技引用本文复制引用
刘万,张晓臣,赵春暖,张浩瑜,杨李俊淞,韩怡静,高雁,王晓通..microRNA-183-5p通过调控MITF基因影响长牡蛎黑色素形成[J].海洋科学,2025,49(7):63-72,10.基金项目
国家自然科学基金资助项目(42076088) （42076088）
山东省自然科学基金资助项目(ZR2023QC107,ZR2022QC233) （ZR2023QC107,ZR2022QC233）
山东省重点研发计划资助项目(2022LZGC015) （2022LZGC015）
山东省泰山学者专项资金项目(tstp20240518) （tstp20240518）
山东省现代农业产业技术体系项目(SDAIT-14-01) （SDAIT-14-01）
烟台市省级以上领军人才专项支持资金项目(202203) （202203）
烟台市水产种业提升项目(2023HD)The National Natural Science Foundation of China,No.42076088 （2023HD）
The Natural Science Foundation of Shandong Province,Nos.ZR2023QC107,ZR2022QC233 （）
The Key Research and Development Program of Shandong Province,No.2022LZGC015 （）
The Special Funds for Taishan Scholars of Shandong Province,No.tstp20240518 （）
The Mod-ern Agricultural Industry Technology System of Shandong Province,No.SDAIT-14-01 （）
The Special Supporting Funds for Leading Talents above Provincial Level in Yantai City,No.202203 （）
The Aquaculture Seed Indus-try Promotion Project of Yantai City,No.2023HD （）
