家畜生态学报2025，Vol.46Issue(12)：15-23,9.DOI:10.3969/j.issn.1673-1182.2025.12.003
鼠伤寒沙门氏菌对文昌鸡盲肠转录组及其肠道菌群的影响
Effect of Salmonella typhimurium on the Cecum Transcriptome and Its Intestinal Flora of Wenchang Chickens
摘要
Abstract
Salmonella typhimurium,as one of the major intestinal pathogens in poultry,interacts with host intestinal cells and the gut microbiota to facilitate its own invasion and colonization after infection.To investigate the mechanisms underlying its interactions with the gut microbiota and the host,this experi-ment infected 14-day-old Wenchang chickens with S.typhimurium.Changes in the cecal microbiota struc-ture and host gene expression were comparatively analyzed using 16S rRNA sequencing and transcriptome sequencing technologies.The results showed that,following S.typhimurium infection,the overall diver-sity of the cecal microbiota in Wenchang chickens showed no significant difference compared to the non-in-fected group(P＞0.05).Compared to the healthy control group,the relative abundance of the phylum Fir-micutes in the infected group decreased by 2.98％,whereas the abundance of its subordinate families Ru-minococcaceae and Veillonellaceae increased significantly(P＜0.05).Simultaneously,the abundance of Coriobacteriaceae,belonging to the phylum Actinobacteria,also showed a significant increase(P＜0.05).Transcriptome analysis further revealed that the downregulated differentially expressed gene PLA2G12B was enriched in metabolic pathways such as arachidonic acid and linolenic acid metabolism,with the arachidonic acid metabolic pathway being closely associated with the initiation of inflammatory responses.The findings of this study provide new insights into the relationships among S.typhimurium,the gut mi-crobiota,and poultry.Further in-depth research will contribute to the treatment of salmonellosis.关键词
文昌鸡/鼠伤寒沙门氏菌感染/肠道菌群/转录组测序分析Key words
Wenchang chicken/Salmonella typhimurium infection/intestinal flora/transcriptome se-quencing analysis分类
农业科技引用本文复制引用
谢尧辰,陈胜宏,闻晓波,冉旭华..鼠伤寒沙门氏菌对文昌鸡盲肠转录组及其肠道菌群的影响[J].家畜生态学报,2025,46(12):15-23,9.基金项目
海南省自然科学基金项目(321RC1020) （321RC1020）
国家自然科学地区基金项目(32360878) （32360878）
