家畜生态学报2025，Vol.46Issue(12)：65-71,7.DOI:10.3969/j.issn.1673-1182.2025.12.010
TLR7/8激活剂R848通过IKBA/ACC/CPT1/FAO途径抑制奶牛精子活力
TLR7/8 Activator R848 Inhibits Sperm Motility in Dairy Cows via the IKBA/ACC/CPT1/FAO Pathway
摘要
Abstract
The aim of this study was to analyze the effect of Toll-like receptor 7/8(TLR7/8)activator R848 on sperm function in dairy cows and to preliminarily investigate its mechanism of action.After co-in-cubation of Holstein cow semen with R848,sperm motility performance and sperm quality parameters were analyzed and the expression levels of fatty acid β-oxidation(FAO)-related proteins were detected by Western blot.The results showed that after co-incubation with R848,the proportion of spermatozoa that swam to the upper layer was significantly lower than that of the control group(P＜0.05),and the kinetic parameters of the lower spermatozoa(VSL,VCL,VAP)were significantly lower than that of the control group and the upper spermatozoa(P＜0.05),whereas there was no significant difference between sperm viability and acrosomal and plasma membrane intact rates(P＞0.05).Western blot results showed that the expression levels of IKBA and ACC proteins in the spermatozoa of dairy cows in the R848 co-incubation group were significantly higher,whereas the expression level of CPT1 protein was significantly lower com-pared with the control group(P＜0.05).The results showed that the TLR7/8 activator R848 could inhibit cow's sperm viability without affecting sperm function by reducing cow sperm ATP production through the IKBA/ACC/CPT1/FAO pathway.关键词
TLR7/8/R848/奶牛精子/FAO/精子活力Key words
TLR7/8/R848/dairy cow spermatozoa/FAO/sperm motility分类
农业科技引用本文复制引用
朱大伟,卫利选,牛隽,曹平,贾志宏,胡建宏..TLR7/8激活剂R848通过IKBA/ACC/CPT1/FAO途径抑制奶牛精子活力[J].家畜生态学报,2025,46(12):65-71,7.基金项目
陕西省农业领域一般项目(2023-YBNY-139),西安市科技计划项目(21NYYF0014) （2023-YBNY-139）
