基础医学与临床2026，Vol.46Issue(1)：1-4,4.DOI:10.16352/j.issn.1001-6325.2026.01.0001
小鼠骨髓来源巨噬细胞原代培养中NG2表达及生物活性
NG2 expression in primary culture of bone marrow derived-macrophages and its bioactivity
摘要
Abstract
Objective To study the neuron-glial antigen 2(NG2)-positive macrophages and their bioactivity in pri-mary cultured mouse bone marrow derived macrophages.Methods Bone marrow single cell suspension was isolated from femur of 6-8 weeks old Kunming mouse co-cultured with L929 supernatant.NG2 expression was detected by immuno-fluorescence staining microscopy at 1,3,5 and 7 days.The proliferation and phagocytosis of NG2-positive macrophages were examined by immunofluorescence staining microscopy and EdU labeling.Results The expression of NG2 markers and positive rate of EdU in NG2-positive macrophages all increased gradually with the prolongation of culture time(P＜0.05).Conclusions NG2-positive macrophages from mouse bone marrow show significant proliferation and phagocytosis function.关键词
巨噬细胞/神经元-胶质抗原2(NG2)/增殖/吞噬Key words
macrophage/neuron-glial antigen 2(NG2)/proliferation/phagocytosis分类
医药卫生引用本文复制引用
程志坚,王国毓,贺西京,翟静静,王涛宁..小鼠骨髓来源巨噬细胞原代培养中NG2表达及生物活性[J].基础医学与临床,2026,46(1):1-4,4.基金项目
陕西省重点研发计划(2023-YBSF-192) （2023-YBSF-192）
陕西省卫生健康科研项目(2021D011) （2021D011）
西安市创新能力强基计划(22YXYJ0144,2021ZDYF-SF-0052) （22YXYJ0144,2021ZDYF-SF-0052）
陕西省中医药大学基金(2021FS7) （2021FS7）
