摘要
Abstract
Objective:To investigate the effect of miR-488-3p targeting pentraxin 3(PTX3)on the osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).Methods:QRT-PCR was used to detect the expression of miR-488-3p and PTX3 mRNA in undifferentiated and differentiated BMSCs.BMSCs were divided into seven groups:control group,inhibition control group,miR-488-3p inhibition group,overexpression control group,PTX3 overexpression group,miR-488-3p inhibition+si-NC group,and miR-488-3p inhibition+si-PTX3 group.QRT-PCR was applied to detect the expression of miR-488-3p and the mRNA expression of PTX3,Runt-related transcription factor 2(Runx2),osterix,osteocalcin(OCN),and osteopontin(OPN)in BMSCs.CCK-8 was used to detect the viability of BMSCs.The activity of alkaline phosphatase(ALP)and the degree of ALP staining in BMSCs were detected.Alizinin red staining was adopted to detect calcium deposition levels and OD570 in BMSCs.Western blotting was employed to detect the protein expression levels of Runx2,osterix,OCN,OPN,and PTX3.Additionally,the relationship between miR-488-3p and PTX3 was verified.Results:In differentiated BMSCs,the expression of miR-488-3p was downregulated,while the expression of PTX3 mRNA was upregulated.Either inhibiting miR-488-3p or overexpressing PTX3 significantly increased the expression level of PTX3 mRNA,BMSC viability,ALP activity,degree of ALP staining,calcium deposition level,OD570,as well as the mRNA and protein expression levels of Runx2,osterix,OCN,and OPN in BMSCs.Silencing PTX3 reversed the promoting effect of miR-488-3p inhibition on the osteogenic differentiation of BMSCs.Moreover,miR-488-3p was found to target and regulate the expression of PTX3.Conclusion:Inhibition of miR-488-3p may promote osteogenic differentiation of BMSCs by upregulating of PTX3.关键词
miR-488-3p/正五聚蛋白3/骨质疏松症/骨髓间充质干细胞/成骨分化Key words
miR-488-3p/pentraxin 3/osteoporosis/bone marrow mesenchymal stem cell/osteogenic differentiation分类
医药卫生
