解剖学杂志2025，Vol.48Issue(6)：487-491,5.DOI:10.3969/j.issn.1001-1633.2025.06.007
异丙酚对大鼠成骨细胞成熟分化的影响
Effects of propofol on osteogenic differentiation of rat osteoblasts
摘要
Abstract
Objective:To investigate the effect of propofol on osteogenic differentiation of rat osteoblasts and its possible mechanism.Methods:Osteoblasts isolated from neonatal SD rat calvariae were induced to differentiate and were divided into control(differentiation induction only),propofol(12.5,25.0,and 50.0 μmol/L),and propofol+phosphatidylinositol 3-kinase(PI3K)inhibitor(LY294002)groups(50.0 μmol/L propofol+10.0 μmol/L LY294002).After 7 d of culture,RT-qPCR was used to detect the mRNA expression levels of alkaline phosphatase(ALP),osteocalcin(OCN),osteopontin(OPN),Runt-related transcription factor 2(RUNX2),and osterix(Osx)genes in osteoblasts.The expressions of OCN,OPN,RUNX2 and Osx proteins were detected by immunofluorescence staining.Western blotting was performed to detect PI3K,p-PI3K,protein kinase B(AKT),p-AKT,and B-cell lymphoma-extra large(Bcl-xL)proteins.ALP staining was adopted after 14 days of cultivation to detect the positivity rate of ALP in rat osteoblasts.After 21 days of cultivation,Alizarin red staining was applied to detect the mineralized nodule formation of rat osteoblasts.Results:After the treatment of propofol with different concentrations,the ALP-positive rate of osteoblasts,mineralized nodule formation,mRNA levels of ALP,OCN,OPN,RUNX2 and Osx,immunofluorescence intensity of OCN,OPN,RUNX2 and Osx,as well as the ratios of p-PI3K/PI3K and p-AKT/AKT and the expression of Bcl-xL were increased.LY294002 attenuated the effects of 50.0 μmol/L propofol on osteogenic differentiation of rat osteoblasts.Conclusion:Propofol promotes osteogenic differentiation of rat osteoblasts,and its mechanism of action may be associated with the activation of the PI3K/AKT/Bcl-xL signaling pathway.关键词
成骨细胞/成骨分化/异丙酚/磷脂酰肌醇3激酶/蛋白激酶BKey words
osteoblast/osteogenic differentiation/propofol/phosphatidylinositol 3-kinase/protein kinase B分类
医药卫生引用本文复制引用
王立,陈俊峰,杨永强..异丙酚对大鼠成骨细胞成熟分化的影响[J].解剖学杂志,2025,48(6):487-491,5.基金项目
2020年佛山市卫生局课题(20200307) （20200307）
