实用临床医药杂志2025，Vol.29Issue(24)：12-18,29,8.DOI:10.7619/jcmp.20253634
非小细胞肺癌组织中泛素特异性肽酶15表达与临床病理特征及预后的关系
Relationships of the expression of ubiquitin-specific peptidase 15 in non-small cell lung cancer tissues with clinicopathological features and prognosis
摘要
Abstract
Objective To investigate the expression of ubiquitin-specific peptidase 15(USP15)in cancerous and adjacent non-cancerous tissues of patients with non-small cell lung cancer(NSCLC)and its relationships with clinicopathological features and prognosis.Methods Cancerous tissue sam-ples and paired adjacent non-cancerous tissue samples were collected from 137 NSCLC patients who underwent surgical treatment.Reverse transcription real-time quantitative polymerase chain reaction(RT-qPCR),Western blotting(WB),and immunohistochemistry(IHC)were used to detect the ex-pression of USP15 in cancerous and adjacent non-cancerous tissues.Kaplan-Meier survival curves were plotted,and the Log-rank test was used to compare survival differences among patients with dif-ferent USP15 protein expression levels.Receiver operating characteristic(ROC)curves were plotted to evaluate the predictive value of USP15 for the prognosis of NSCLC patients.Univariate and multi-variate Cox regression analyses were conducted to screen the factors influencing the prognosis of NSCLC patients.Results The results of RT-qPCR and WB showed that compared with adjacent non-cancerous tissues,the expression levels of USP15 mRNA and USP15 protein in lung adenocarcinoma and lung squamous cell carcinoma tissues were increased,with statistically significant differences(P＜0.05).The results of IHC showed that the high expression rate of USP15 in cancerous tissues of NSCLC patients was higher than that in adjacent non-cancerous tissues,with a statistically signifi-cant difference(P＜0.05).The expression levels of USP15 in cancerous tissues of NSCLC patients with a maximum tumor diameter≥3 cm,lymph node metastasis,TNM stage Ⅲ to Ⅳ,and low dif-ferentiation were respectively higher than those in patients with a maximum tumor diameter＜3 cm,no lymph node metastasis,TNM stage Ⅰ to Ⅱ,and moderate-to-high differentiation,with statisti-cally significant differences(P＜0.05).Kaplan-Meier survival curve analysis showed that during a 3-year follow-up,the median survival time of patients with high USP15 expression was 12.2 months,which was shorter than the 30.4 months of patients with low USP15 expression,with a statistically significant difference(Log-rank x2=7.664,P=0.006).During a 5-year follow-up,the median survival time of patients with high USP15 expression was 27.4 months,which was shorter than the 50.6 months of the low USP15 expression group,with a statistically significant difference(Log-rank x2=54.359,P＜0.001).ROC curve analysis showed that the area under the curve for USP15 in predicting the prognosis of NSCLC patients was 0.955.Multivariate Cox regression analysis showed that lymph node metastasis,low differentiation,TNM stage Ⅲ-Ⅳ,and high USP15 expression were independent risk factors for poor prognosis in NSCLC patients during a 3-year follow-up(P＜0.05).Conclusion USP15 is upregulated in cancerous tissues of NSCLC patients,and its expres-sion is related to lymph node metastasis,TNM stage,and differentiation.High USP15 expression is an independent risk factor for poor prognosis in NSCLC patients.关键词
非小细胞肺癌/泛素特异性肽酶15/临床病理特征/预后/TNM分期/生物标志物/淋巴结转移/分化程度Key words
non-small cell lung cancer/ubiquitin-specific peptidase 15/clinicopathological features/prognosis/TNM stage/biomarker/lymph node metastasis/differentiation分类
医药卫生引用本文复制引用
杨嘉麟,谢伶俐,林安琪..非小细胞肺癌组织中泛素特异性肽酶15表达与临床病理特征及预后的关系[J].实用临床医药杂志,2025,29(24):12-18,29,8.基金项目
广东省中医药局科研项目(20202191) （20202191）
