江西农业学报2026，Vol.38Issue(1)：1-7,61,8.DOI:10.19386/j.cnki.jxnyxb.2026.01.001
基于SNP遗传图谱定位甘蓝型油菜氮肥高效吸收相关性状的QTLs
Mapping QTLs Associated with Nitrogen Uptake Efficiency Traits in Rapeseed Based on SNP Genetic Map
摘要
Abstract
This study utilized two distinct rapeseed varieties,Xiangyou 15 with high nitrogen fertilizer absorption efficiency and R210 with low nitrogen fertilizer absorption efficiency,to produce an F1 hybrid.A doubled haploid(DH)population comprising 180 lines was obtained through microspore culture.Phenotypic investigation and QTL analysis were conducted on traits closely related to nitrogen fertilizer absorption efficiency in rapeseed under two nitrogen fertilization levels,including basal stem diameter,effective pod number per plant,aboveground dry weight per plant,and nitrogen absorption efficiency.The results showed that a high-density genetic map was constructed,covering 19 chromosomes of rapeseed,comprising 42751 polymorphic SNP markers,4302 bins,with a genome coverage of 2959.89 cM and an average interval of 0.69 cM between adjacent bins.Based on this map,using the composite interval mapping method(CIM),14 QTLs were detected under two nitrogen application levels,with individual QTL explaining 5.24%to 18.22%of the phenotypic variation.Seven candidate genes and one p-loop containing nucleoside triphosphate hydrolases superfamily protein of chloroplastic-like potentially associated with nitrogen use efficiency traits in rapeseed were identified.These QTLs and candidate genes will provide valuable information for the genetic improvement of nitrogen use efficiency in rapeseed.关键词
甘蓝型油菜/氮肥吸收效率/QTL位点/候选基因Key words
Rapeseed(Brassica napus L.)/Nitrogen fertilizer absorption efficiency/QTLs/Candidate gene分类
农业科技引用本文复制引用
邹小云,闫蕾,黄杨..基于SNP遗传图谱定位甘蓝型油菜氮肥高效吸收相关性状的QTLs[J].江西农业学报,2026,38(1):1-7,61,8.基金项目
国家自然科学基金(31760393、32060436) （31760393、32060436）
江西省"双千计划"科技创新高端人才项目(jxsq2019201013) （jxsq2019201013）
江西省杰出青年人才资助计划(20171BCB23082). （20171BCB23082）
