解放军医学院学报2025，Vol.46Issue(10)：954-959,6.DOI:10.12435/j.issn.2095-5227.24082002
NF-κB信号通路通过影响子宫trNK细胞募集致小鼠子代生长受限
NF-κB signaling pathway impairs fetal growth in mice by regulating recruitment of uterine trNK cells
摘要
Abstract
Background The nuclear factor kappa-B(NF-κB)pathway is involved in regulating the expression of chemokines,inflammatory cytokines and matrix metalloproteinases,and plays an important role in decidualization and embryo implantation.Objective To investigate the effects of NF-κB activity on the growth of mouse and its possible mechanism.Methods Thirty-two ICR mice were randomly divided into two groups,all of which underwent natural pregnancy.The experimental group received intraperitoneal injections of NF-KB inhibitor dissolved in PBS from gestational days 5.5 to 7.5,while the control group received intraperitoneal injections of the same volume of PBS during the same period.The conceptuses of the two groups were weighed at 8.5 days of gestation(n=10).On day 14.5(n=6),the weight of offspring and placentas were weighed and placental efficiency(placenta weight/fetal weight)was calculated.Immunohistochemistry was used to detect the effect of NF-κB inhibitor on the number of trNK cells in the uterus of mice.Real time PCR was used to detect the expression of chemokines and cytokines related to NK cell recruitment and differentiation in the decidua.Results NF-κB inhibitor administration significantly reduced the weights of implantation sites at gestation day 8.5(P＜0.001),as well as fetal weight(P＜0.001),placental weight(P＜0.05),and placental efficiency(P＜0.01)at gestation day 14.5.At gestation day 8.5,APDC-treated mice exhibited a significantly reduced density of uterine trNK cells(proportion of immunopositive cells;P＜0.001).Furthermore,APDC treatment significantly decreased the expression levels of decidual chemokines and cytokines(P＜0.05).Conclusion Inhibition of NF-κB activity can reduce the expression of chemokines and cytokines in the decidua of mice,and then affect the recruitment and differentiation of uterine trNK cells,resulting in growth restriction of offspring.关键词
NF-κB活性/子宫trNK细胞/细胞因子/趋化因子/生长Key words
NF-κB activity/uterine trNK cells/cytokines/chemokine/growth分类
医药卫生引用本文复制引用
马蓉,陈书强,金妮,王晓红..NF-κB信号通路通过影响子宫trNK细胞募集致小鼠子代生长受限[J].解放军医学院学报,2025,46(10):954-959,6.基金项目
国家自然科学基金面上项目(82271734 （）
82071717) （）
