检验医学与临床2026，Vol.23Issue(1)：1-6,6.DOI:10.3969/j.issn.1672-9455.2026.01.001
超声参数联合血清miR-129-5p、miR-654-5p对乳腺癌的诊断价值
Diagnostic value of ultrasound combined with serum miR-129-5p and miR-654-5p in breast cancer
摘要
Abstract
Objective To investigate the diagnostic value of ultrasound combined with serum miR-129-5p and miR-654-5p in breast cancer.Methods A total of 93 female patients with breast cancer who underwent surgical treatment in Shiyan Taihe Hospital of Hubei Province from December 2021 to December 2023 were selected as the breast cancer group,93 female patients with benign breast lesions were selected as the benign group,and 93 female healthy subjects who underwent physical examination in Shiyan Taihe Hospital of Hubei Province during the same period were selected as the control group.All subjects underwent ultrasound exami-nation,and the related parameters[maximum blood flow velocity(Vmax),resistance index(RI),pulsatility index(PI)]were recorded.The levels of serum miR-129-5p and miR-654-5p in all subjects were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction.The receiver operating char-acteristic(ROC)curve was drawn to analyze the diagnostic value of Vmax,RI,PI combined with serum miR-129-5p and miR-654-5p in breast cancer.Results The serum levels of miR-129-5p and miR-654-5p in the be-nign group and the breast cancer group were significantly lower than those in the control group,and the serum levels of miR-129-5p and miR-654-5p in the breast cancer group were lower than those in the benign group,and the differences were statistically significant(P<0.05).The Vmax,RI and PI of the benign group and the breast cancer group were significantly higher than those of the control group,and the Vmax,RI and PI of the breast cancer group were higher than those of the benign group,and the differences were statistically signifi-cant(P<0.05).The low expression rates of serum miR-129-5p and miR-654-5p in breast cancer patients with TNM stage Ⅲ-Ⅳ,lymph node metastasis and moderate and low differentiation were higher than those in breast cancer patients with TNM stage Ⅰ-Ⅱ,no lymph node metastasis and high differentiation,and the differences were statistically significant(P<0.05).ROC curve analysis showed that the area under the curve(AUC)of the combination of Vmax,RI,PI,miR-129-5p and miR-654-5p in the diagnosis of breast cancer was 0.892,which was greater than the AUC of the five alone(0.712,0.783,0.720,0.648,0.718),the differences were all statistically significant(Z=4.013,4.215,3.889,6.223,3.887,P<0.05).Conclusion The levels of serum miR-129-5p and miR-654-5p in breast cancer patients were decreased,and the ultrasound(Vmax,RI,PI)were increased.Ultrasound parameters combined with serum miR-129-5p and miR-654-5p have high diagnostic value for breast cancer.关键词
超声参数/miR-129-5p/miR-654-5p/乳腺癌/诊断价值/微小RNAKey words
ultrasound parameter/miR-129-5p/miR-654-5p/breast cancer/diagnostic value/mi-croRNA分类
医药卫生引用本文复制引用
代妮娜,张文君,张华..超声参数联合血清miR-129-5p、miR-654-5p对乳腺癌的诊断价值[J].检验医学与临床,2026,23(1):1-6,6.基金项目
湖北省科技厅自然科学基金创新发展联合基金项目(2024AFD092). （2024AFD092）
