检验医学与临床2026，Vol.23Issue(1)：13-19,7.DOI:10.3969/j.issn.1672-9455.2026.01.003
肾透明细胞癌患者SALL4、Bmi-1蛋白水平及其与上皮-间质转化的关系
Levels of SALL4 and Bmi-1 protein in patients with renal clear cell carcinoma and their relationship with epithelial-mesenchymal transition
摘要
Abstract
Objective To investigate the levels of sala-like gene 4(SALL4)and B cell-specific moloney leu-kemia virus insertion site 1(Bmi-1)protein in patients with renal clear cell carcinoma and their relationship with epithelial-mesenchymal transition(EMT).Methods A total of 87 patients with renal clear cell carcino-ma after surgical treatment admitted to the Department of Oncology of the hospital from March 2020 to March 2024 were selected as the research objects.The protein expression of EMT markers(E-cadherin,N-cad-herin,vimentin)in cancer tissues and adjacent tissues of patients was detected by immunohistochemistry(re-ferred to as immunohistochemistry),and the staining score was calculated.The protein levels of SALL4,Bmi-1 and EMT markers in cancer tissues and adjacent tissues were detected by Western blotting.According to the mean value of SALL4 and Bmi-1 protein levels in cancer tissues of all patients,the patients were divided into high SALL4 level group(≥mean value)and low SALL4 level group(<mean value),high Bmi-1 level group(≥mean value)and low Bmi-1 level group(<mean value).Pearson correlation was used to analyze the corre-lation between E-cadherin,N-cadherin,vimentin and SALL4,Bmi-1 protein levels in cancer tissues of patients with renal clear cell carcinoma.Receiver operating characteristic(ROC)curve was used to analyze the predic-tive value of SALL4 and Bmi-4 protein in cancer tissues for EMT in patients with renal clear cell carcinoma.The differences of E-cadherin,N-cadherin and vimentin levels in patients with different SALL4 and Bmi-4 lev-els were compared.Results The results of immunohistochemical detection showed that the immunohisto-chemical staining score and positive rate of protein expression of E-cadherin in cancer tissues were lower than those in adjacent tissues(P<0.001),and the immunohistochemical staining score and positive rate of protein expression of N-cadherin and vimentin were higher than those in adjacent tissues(P<0.001).The results of Western blotting showed that the level of E-cadherin protein in cancer tissues was lower than that in adjacent tissues(P<0.001),and the levels of N-cadherin,vimentin,SALL4 protein and Bmi-1 protein were higher than those in adjacent tissues(P<0.001).Pearson correlation analysis showed that the protein levels of SALL4 and Bmi-1 in renal clear cell carcinoma tissues were negatively correlated with the levels of E-cadherin(r=-0.656,-0.654,P<0.001),and positively correlated with the levels of N-cadherin protein and vimen-tin(r=0.636,0.635,0.668,0.674,P<0.001).The immunohistochemical staining scores and levels of E-cad-herin in cancer tissues of high SALL4 level group were lower than those of low SALL4 level group,and the immunohistochemical staining scores and protein levels of N-cadherin and vimentin in cancer tissues of high SALL4 level group were higher than those of low SALL4 level group,and the differences were statistically significant(P<0.05).The immunohistochemical staining score and protein level of cancer tissue protein in the high Bmi-1 level group were lower than those in the low Bmi-1 level group,and the immunohistochemical staining score and protein level of N-cadherin and vimentin were higher than those in the low Bmi-1 level group,and the differences were statistically significant(P<0.05).The protein levels of SALL4 and Bmi-1 from cancer tissue in the EMT group were higher than those in the non-EMT group,and the differences were statistically significant(P<0.05).The results of ROC curve analysis showed that the area under the curve(AUC)of SALL4 and Bmi-1 protein combined in cancer tissues to predict EMT in patients with renal clear cell carcinoma was 0.849,which was greater than the AUC of SALL4 and Bmi-1 protein predicted alone(0.700,0.724),and the differences were statistically significant(Z=3.121,3.014,P<0.05).Conclusion The pro-tein levels of SALL4 and Bmi-1 in renal clear cell carcinoma tissues are significantly increased,and they are re-lated to the occurrence of EMT,suggesting that SALL4 and Bmi-1 protein may be important targets for the progression of renal clear cell carcinoma.关键词
肾透明细胞癌/婆罗双树样基因4/B细胞特异性莫洛尼氏白血病毒插入位点1/上皮-间质转化Key words
renal clear cell carcinoma/sala-like gene 4/B-cell-specific moloney leukemia virus inser-tion site 1/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
陈敏,许丹妮,吴仁正,夏威夷..肾透明细胞癌患者SALL4、Bmi-1蛋白水平及其与上皮-间质转化的关系[J].检验医学与临床,2026,23(1):13-19,7.基金项目
海南省卫生健康行业科研项目(19A200504) （19A200504）
海南医科大学学术提升支撑计划(XSTS2025112). （XSTS2025112）
