辽宁中医药大学学报2026，Vol.28Issue(1)：29-33,5.DOI:10.13194/j.issn.1673-842X.2026.01.006
水晶兰苷对APP-N2a细胞神经保护作用及机制研究
Neuroprotective Effect and Mechanism of Monotropein on APP-N2a Cells
摘要
Abstract
Objective To investigate the neuroprotective and mechanism of monotropein(Mon)on the N2a cell infected by adenoviral carrying the APPswe gene.Methods The CCK-8 assay was used to detect the optimal concentration by Mon and Nrf2 activator(Nrf2-Ac)on APP-N2a cells,flow cytometry was used to detect the effect of Mon and Nrf2-Ac on apoptosis of APP-N2a cells,ELISA assay was used to detect the effect of Mon and Nrf2-Ac on inflam-cytokines of APP-N2a cells and immunohistochemistry assay was used to detect the effect of Mon and Nrf2-Ac on Nrf2,HO-1 protein expression of APP-N2a cells.Results The results showed that 20 μM Mon and 1000 nM Nrf2-Ac could increase the survival rate and reduce apoptosis of the APP-N2a cells.The levels of IL-6,IL-1β,TNF-α were decreased while the level of Nrf2,HO-1 protein expression was increased.Conclusion Mon has the most significant neuroprotective in the APP-N2a cells by activating the Nrf2/HO-1 signaling pathway.关键词
水晶兰苷/APP-N2a细胞/神经保护作用/Nrf2/HO-1信号通路Key words
monotropein/APP-N2a cells/neuroprotective/Nrf2/HO-1 signaling pathway分类
医药卫生引用本文复制引用
李雪彧,王威丽,符宁宁,吴运谱,宁天一,张泽辉,王健,崔子寅,梁喜才..水晶兰苷对APP-N2a细胞神经保护作用及机制研究[J].辽宁中医药大学学报,2026,28(1):29-33,5.基金项目
辽宁省教育厅面上项目(LJKZ0892,2024-JYTCB-103) （LJKZ0892,2024-JYTCB-103）
辽宁省科技计划联合计划(应用基础研究项目)项目(2023JH2/101700213) （应用基础研究项目）
辽宁中医药大学高层次人才科研启动基金项目(2100223016) （2100223016）
