麦类作物学报2026，Vol.46Issue(1)：90-100,11.DOI:10.7606/j.issn.1009-1041.2026.01.10
大麦LRR型类受体蛋白激酶基因HvLRR-RLK510启动子的克隆与功能验证
Cloning and Functional Validation of the Promoter of the LRR-Type Receptor-Like Protein Kinase Gene HvLRR-RLK510in Barley
摘要
Abstract
To elucidate the promoter of the barley LRR-type receptor-like protein kinase gene HvLRR-RLK510 and its function,specific PCR primers were used to amplify the promoter of the HvLRR-RLK510 gene from Morex and Baodamai 8.The pEASY®-510 recombinant plasmid was constructed,and the promoter sequence of 2 368 bp was sequenced based on the positive colonies of the Escherichia coli Trans1-T1 strain.The prediction results from the online software PlantCARE indicated that the promoter contains response elements to non-biotic factors such as methyl jasmonate,abscisic acid,and low temperature.GUS staining results in roots,stems,leaves,and flowers of transgenic Arabi-dopsis thaliana and transiently expressed Nicotiana benthamiana plants demonstrated that the cloned promoter and its truncated fragments(-1 458,-868,and-460 bp)can equivalently drive the ex-pression of the GUS gene in anthers.Quantitative real-time PCR(qRT-PCR)results showed that methyl jasmonate(MeJA),abscisic acid(ABA),low temperature,polyethylene glycol(PEG-2000),and light can effectively alter the expression characteristics of the HvLRR-RLK510 gene in barley leaves and roots.The promoter of the HvLRR-RLK510 gene cloned in this study is an inducible pro-moter containing response elements to jasmonic acid,abscisic acid,low temperature,drought,and light.Its-460 bp truncated fragment can replace the full-length promoter.The promoter and its truncated fragments can be used to elucidate the transcriptional regulatory mechanisms of the HvL-RR-RLK510 gene.关键词
大麦/HvLRR-RLK510基因/启动子克隆/遗传转化/功能验证Key words
Hordeum vulgare L./HvLRR-RLK510 gene/Promoter/Genetic transformation/Func-tional validation分类
农业科技引用本文复制引用
董陈文华,李勇,王玫郦,陈升位,周洪斌,郎雨萌,罗亮扎,李仕金,姜瑞梅,毛孝强,赵海云,唐进..大麦LRR型类受体蛋白激酶基因HvLRR-RLK510启动子的克隆与功能验证[J].麦类作物学报,2026,46(1):90-100,11.基金项目
国家自然科学基金项目(32360469) （32360469）
云南省教育厅科学研究基金项目(2025Y0498) （2025Y0498）
