中南医学科学杂志2026，Vol.54Issue(1)：25-29,5.DOI:10.15972/j.cnki.43-1509/r.2026.01.005
POMC基因敲除对高脂饮食诱导的肥胖雄性幼鼠睾丸功能的影响机制
The mechanism of POMC gene knockout to affect testicular function in obese male young mice induced by high-fat diet obesity
摘要
Abstract
Aim To explore the mechanism of pro-opiomelanocortin(POMC)gene knockout to affect testicular function in obse young mice induced by high-fat diet obesity.Methods Twenty wild-type C57BL/6 male offspring mice were randomly and equally divided into a control group(fed a standard diet)and a high-fat diet group(fed a high-fat diet),while another ten POMC-/-pups were used as the knockout POMC high-fat group(fed a high-fat diet).The body weight of neonatal mice among different groups at the same time were compared,the testicular volume was measured.HE staining and oil red O staining were used to detect the his-topathological structure and fat content of testicular tissues in each group;Western blotting and qRT-PCR were used to detect the pro-tein and mRNA expression levels of POMC and cocaine-and amphetamine-regulated transcript(CART)in testicular tissues.The re-agent kit is used to detect the levels of serum testosterone and blood lipids[triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDLC),and high-density lipoprotein cholesterol(HDLC)];TUNEL staining was used to detect the apoptosis level of testicular tissue cells in each group.Results Compared with the control group,the high-fat diet group and the knockout POMC high-fat group showed an increase in body weight at weeks 8 and 12,and red-stained neutral fat in testicular tissue(P<0.05),while a decrease in germ cell layer of seminiferous tubules in testicular tissue(P<0.05),a decrease in testicular volume,testoster-one,HDLC,and POMC,CART protein and their mRNA expression levels(P<0.05),and an increase inTC,TG,LDLClevels and cell apoptosis levels(P<0.05).Compared with the high-fat diet group,the weight at weeks 8 and 12,and red-stained neutral fat in testicular tissue of the knockout POMC high-fat group were increased(P<0.05),while the germ cell layer of seminiferous tubules in testicular tissue were decreased(P<0.05),and testicular volume,testosterone,HDLC,and POMC,CART protein and their mRNA levels were also decreased(P<0.05),in addition,TC,TG,LDLC and cell apoptosis levels were increased(P<0.05).Conclu-sion Knockout of POMC gene significantly exacerbates testicular dysfunction induced by high-fat diet in obese male young mice,and its mechanism may be related to the inhibition of POMC/CART signaling pathway,leading to metabolic disorders and increased cell apoptosis.关键词
肥胖/POMC基因敲除/睾丸功能/高脂饮食/幼鼠Key words
obesity/POMC gene knockout/testicular function/high-fat diet/young mice分类
医药卫生引用本文复制引用
龙海旭,詹红艳,狄雯雯,唐美美..POMC基因敲除对高脂饮食诱导的肥胖雄性幼鼠睾丸功能的影响机制[J].中南医学科学杂志,2026,54(1):25-29,5.基金项目
湖南省卫生健康委卫生科研课题项目(W20243267) （W20243267）
