中南医学科学杂志2026，Vol.54Issue(1)：30-34,5.DOI:10.15972/j.cnki.43-1509/r.2026.01.006
熊果酸调控VEGF/COX-2/MMP-2通路减轻糖尿病小鼠视网膜病变损伤
Ursolic acid alleviates retinal injury in diabetic mice by regulating the VEGF/COX-2/MMP-2 pathway
摘要
Abstract
Aim To investigate the beneficial effects of ursolic acid(UA)to alleviate retinal injury in diabetic mice through the mechanism of regulating vascular endothelial growth factor(VEGF)/cyclooxygenase-2(COX-2)/matrix metalloproteinase-2(MMP-2)pathway.Methods A diabetic retinopathy(DR)mouse model was established and divided into four groups.The UA low-,medium-,and high-dose groups received 10,20,and 40 mg/kg UA by gavage,respectively,while the control and DR groups received equal volumes of normal saline.Retinal endothelial cells and pericytes were counted and their ratio was calculated u-sing PAS staining.Oxidative stress markers(superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px))were measured by immunoprecipitation.Apoptosis-related proteins(Bcl-2,Bax,caspase-3)and VEGF,COX-2,and MMP-2 protein expression were detected by Western blotting.Results Compared with the control group,the DR group showed significantly increased retinal endothelial cell count,endothelial cell/pericyte ratio,MDA content,and protein expression of Bax,cleaved caspase-3,VEGF,COX-2,and MMP-2,while pericyte count,SOD and GSH-Px activities,and Bcl-2 protein expression were significantly decreased(P<0.05).Compared with the DR group,all groups treated with different doses of UA xhibited reduced en-dothelial cell count,endothelial cell/pericyte ratio,MDA content,and protein expression of Bax,cleaved caspase-3,VEGF,COX-2,and MMP-2,with the high-dose group showing the most pronounced effects.Meanwhile,pericyte count,SOD and GSH-Px activities,and Bcl-2 protein expression were significantly increased in a dose-dependent manner(P<0.05).Conclusion UA protects retinal tissue in DR mice in a dose-dependent manner by downregulating the expression of VEGF,COX-2,and MMP-2,provi-ding a new scientific basis for clinical treatment of DR.关键词
熊果酸/糖尿病视网膜病变/VEGF/COX-2/MMP-2Key words
ursolic acid/diabetic retinopathy/vascular endothelial growth factor/cyclooxygenase-2/matrix metallopro-teinase-2分类
医药卫生引用本文复制引用
杨璇,王永强,崔泰国,齐艳秀..熊果酸调控VEGF/COX-2/MMP-2通路减轻糖尿病小鼠视网膜病变损伤[J].中南医学科学杂志,2026,54(1):30-34,5.基金项目
黑龙江省卫生健康委科研课题(2021070720086) （2021070720086）
