北方农业学报2025，Vol.53Issue(5)：36-48,13.DOI:10.12190/j.issn.2096-1197.2025.05.04
154份荞麦荧光特性评价及高光效种质筛选
Evaluation of fluorescence characteristics and screening of high light-use efficiency germplasm in 154 buckwheat varieties
摘要
Abstract
[Objective]To explore the effect of key fluorescence parameters on the characterizing value of high light-use efficiency in buckwheat and to screen high light-use efficiency buckwheat germplasm through fluorescence characteristic evaluation,providing a scientific basis and material support for high light-use efficiency breeding.[Methods]A randomized block field experiment was conducted,using 154 buckwheat varieties as experimental materials.Twenty-five chlorophyll fluorescence parameters were measured.Correlation analysis,principal component analysis(PCA),and systematic clustering-shortest distance analysis were employed to comprehensively evaluate the fluorescence characteristics of buckwheat and identify superior germplasm resources with high light-use efficiency.[Results]Highly significant differences were observed in chlorophyll fluorescence parameters among different buckwheat varieties(P<0.01).Specifically,parameters PI abs、PI total,ETo/CSm,DIo/RC,REo/CSm,Fv/Fo,TRo/CSm,Fv and Fo/Fm showed larger coefficients of variation(CV)values,all exceeding 25.00%.Parameters DF total,DF abs,TRo/RC,DIo/CSm and Fo showed smaller CV values,ranging from 0.71%to 7.97%.Broad-sense heritability of all other parameters,except TRo/RC,REo/RC,REo/CSo and PI total,was greater than 60.00%.Principal component analysis revealed that the cumulative contribution rate of the top four principal components reached 96.758%,and a comprehensive evaluation function model was established as A=0.612A1+0.190A2+0.130A3+0.068A4,with A values for the 154 buckwheat varieties ranging from-2.68 to 1.20.Clustering analysis of the A values classified the tested varieties into five categories:2 buckwheat varieties(NQ148 and NQ150)were classified as the high light-use efficiency germplasm type,105 varieties were classified as the relatively high light-use efficiency germplasm type,44 varieties were classified as the moderate light-use efficiency germplasm type,2 varieties were classified as the relatively low light-use efficiency germplasm type,and 1 variety was classified as the low light-use efficiency germplasm type.[Conclusion]Two buckwheat varieties(NQ148 and NQ150)with high light-use efficiency were successfully screened,providing materials for the breeding of high light-use efficiency germplasm resources in buckwheat.关键词
荞麦/叶绿素/荧光特性/高光效种质/综合评价Key words
Buckwheat/Chlorophyll/Fluorescence characteristics/High light-use efficiency germplasm/Comprehensive evaluation分类
农业科技引用本文复制引用
胡昊驰,吴叶宁,谢锐,刘锦川,温蕊,王志超,王春勇,于传宗,金晓蕾..154份荞麦荧光特性评价及高光效种质筛选[J].北方农业学报,2025,53(5):36-48,13.基金项目
国家重点研发计划项目(2023YFD1600700) （2023YFD1600700）
内蒙古农牧业科研保障经费项目(2023CXJJN05) （2023CXJJN05）
2022年度引进高层次人才科研支持(RZ2400001486) （RZ2400001486）
