家禽科学2026，Vol.48Issue(1)：20-26,后插3,8.
一株肾型鸡传染性支气管炎病毒RP-NR22株的分离鉴定及致病性研究
Isolation,Identification and Pathogenicity Study of A New Renal Type Avian Infectious Bronchitis Virus Strain RP-NR22
段伦涛 1冯敬敬 1段宝敏 1李亚杰 1王一新 2王友1
作者信息
- 1. 天津瑞普生物技术股份有限公司,天津 300308
- 2. 山东农业大学动物医学院,山东 泰安 271018
- 折叠
摘要
Abstract
During February 2022,a contagious illness leading to significant kidney impairment was identified in a flock of white-feathered broiler chickens at a large farm in Shandong Province,China.However,the specific etiological factors remain unclear.In order to ascertain the causative agent of the disease,samples were gathered for RT-PCR testing,virus isolation,and S1 gene sequencing analysis.A nephropathogenic strain of avian infectious bronchitis virus(IBV)was isolated and designated as RP-NR22.Through analysis of genetic evolution centered on the S1 gene,it was determined that this strain is part of the GI-19 lineage and showcases mutations at critical glycosylation sites.In order to explore the pathogenic features of RP-NR22,the virus was administered to SPF chickens to evaluate its pathogenicity.Animal experiments have validated that RP-NR22 induced kidney lesions in all SPF chickens(10/10)and resulted in a mortality rate of 40%(4/10).The commercially available IBV ELISA kits commonly used at present have shown an inability to identify antibody responses in chickens infected with the virus.This report marks the initial identification of a nephropathogenic IBV strain isolated from both domestic and international sources.The findings of this study offer foundational resources and information for advancing research on the pathogenic pathways of nephropathogenic IBV and the development of effective preventive and control measures.关键词
鸡传染性支气管炎病毒/肾病变型/免疫逃逸/动物回归试验Key words
Chicken infectious bronchitis virus/Nephropathogenic type/Immune evasion/Animal pathogenicity experiment分类
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段伦涛,冯敬敬,段宝敏,李亚杰,王一新,王友..一株肾型鸡传染性支气管炎病毒RP-NR22株的分离鉴定及致病性研究[J].家禽科学,2026,48(1):20-26,后插3,8.
