药学研究2025，Vol.44Issue(12)：1145-1152,1179,9.DOI:10.13506/j.cnki.jpr.2025.12.001
赤丹退黄颗粒通过抑制凋亡信号通路改善ANIT诱导胆汁淤积的作用规律
Study of Chidan Tuihuang Granules alleviating ANIT-induced cholestasis by inhibiting apoptosis signaling pathway
摘要
Abstract
Objective To explore the mechanisms by which Chidan Tuihuang Granules(CDTH)improve cholestatic liver injury and to reveal the biological mechanisms that regulate the progression of cholestasis.Methods Using α-naphthyl isothiocyanote(ANIT)-induced cholestasis model,the therapeutic effects of CDTH on cholestasis were evaluated.Network pharmacology and molecular docking techniques were used to identify potential core targets and pathways for CDTH in treating cholestasis.Results Animal experiments confirmed that CDTH significantly reduced liver function indicators and effectively improved liver tissue inflammation and steatosis.Network pharmacology analysis showed that CDTH treatment of cholestasis was significantly enriched in apoptosis signaling pathways.Molecular docking analysis confirmed that the active components of Chidan Tuihuang Granules have a stable binding ability with core targets(binding energy＜-5.0 kcal·mol-1).Conclusion CDTH can effectively improve serum biochemical indicators of ANIT-induced cholestasis,inhibit the expression of apoptosis signaling pathway protein in cholestatic of liver tissue,and thus exert a comprehensive therapeutic effect against cholestasis.关键词
赤丹退黄颗粒/胆汁淤积/分子对接/网络药理学/细胞凋亡Key words
Chidan Tuihuang Granules/Cholestasis/Molecular docking/Network pharmacology/Apoptosis分类
医药卫生引用本文复制引用
曾灵顺,石森蟠,王奕乐,谭玲芝,谭茜月,符玲萍,马骁..赤丹退黄颗粒通过抑制凋亡信号通路改善ANIT诱导胆汁淤积的作用规律[J].药学研究,2025,44(12):1145-1152,1179,9.基金项目
国家自然科学基金面上项目(No.82274187) （No.82274187）
