世界睡眠医学杂志2025，Vol.12Issue(9)：1913-1916,4.DOI:10.3969/j.issn.2095-7130.2025.09.001
松郁安神方对失眠大鼠褪黑素合成及其受体的影响
Effects of Songyu Anshen Prescription on Melatonin Synthesis and Receptor Expression in a Rat Model of Insomnia
摘要
Abstract
Objective:To evaluate whether Songyu Anshen Prescription(SYF)modulates melatonin synthesis and melatonin re-ceptor expression in rats with p-chlorophenylalanine(PCPA)-induced insomnia.Methods:Specific pathogen-free(SPF)male Sprague Dawley(SD)rats were assigned to a control group(n=8)and a model cohort(n=40)rendered insomniac by intraperito-neal PCPA(IP).After model establishment,rats were randomized into five groups(n=8 each):Model(normal saline intragastric gavage,IG),SYF(17 g/kg IG),Luzindole(Luz;1 mg/kg IP),SYF(17 g/kg IG)+Luz(1 mg/kg IP),and Agomelatine(2.25 mg/kg IG).Treatments were administered once daily for 7 days;the control and model groups received an equal volume of normal saline.Serum melatonin was measured by enzyme-linked immunosorbent assay(ELISA).Arylalkylamine N-acetyltrans-ferase(AANAT)in the pineal gland and hypothalamic melatonin receptor 1B(MTNR1B)gene were quantified by western blot-ting.Results:Relative to controls,model rats exhibited significantly lower serum melatonin and reduced protein expression of pine-al AANAT and hypothalamic MTNR1B(P＜0.01).Versus the model group,both the SYF and Agomelatine groups significantly increased serum melatonin and up-regulated pineal AANAT and hypothalamic MTNR1B(P＜0.01).Compared with SYF,Luzin-dole alone and SYF plus Luzindole significantly lowered these measures(P＜0.01),indicating attenuation of SYF's effects by me-latonin receptor antagonism.Conclusion:SYF exhibits melatonin receptor agonist-like activity in PCPA-induced insomnia rats.Its mechanism may involve upregulating pineal AANAT to enhance melatonin synthesis and engaging hypothalamic melatonin recep-tor-mediated signaling.关键词
失眠/疾病模型/动物大鼠/松郁安神方/褪黑素/褪黑素受体/芳香胺-N-乙酰基转移酶/中医药疗法Key words
Insomnia/Disease model/Animal rats/Songyu Anshen Prescription/Melatonin/Melatonin receptor/AANAT/Tra-ditional Chinese Medicine Therapy分类
医药卫生引用本文复制引用
王秀峰,张瑜,张一帆,黄俊山..松郁安神方对失眠大鼠褪黑素合成及其受体的影响[J].世界睡眠医学杂志,2025,12(9):1913-1916,4.基金项目
国家自然科学基金项目(81774285) （81774285）
福建省科学技术厅省属公益类科研院所基本科研专项(2022R1003001) （2022R1003001）
福建省科技厅自然科学基金面上项目(2022J01862) （2022J01862）
