水生生物学报2026，Vol.50Issue(1)：1-9,9.DOI:10.3724/1000-3207.2025.2025.0072
基于代谢组学分析选育系F5代与非选育系马苏大麻哈鱼肌肉代谢物的差异
DIFFERENCES IN MUSCLE METABOLITE BETWEEN SELECTED F5 GENERA-TION AND NON SELECTED ONCORHYNCHUS MASOU BASED ON METABOLOMIC ANALYSIS
摘要
Abstract
The effect of breeding on muscle metabolites of landlocked Oncorhynchus masou was explored by untar-geted metabolomics technology,which provided a theoretical basis for breeding efforts.Muscle tissues were collected from 10 non selected landlocked O.masou and 10 fifth generation(F5)selected individuals,with untargeted metabolomics studies using liquid-mass coupling(LC-MS)technology.Metabolite differences between F5 selected and non-selected groups were compared,with differential metabolites identified using thresholds of P<0.05,VIP value>1.0,and FC≥1.5(up-regulated)or FC≤0.667(down-regulated).Pathway annotation of the metabolites through KEGG database,phenylpropane biosynthesis and metabolic,fat metabolism,and eicosanoids metabolism pathway were analyzed.Significant differences in muscle metabolites were observed between F5 selected and non-selected groups:compared with the non-selected group,213 differential metabolites were significantly up-regulated and 348 were signifi-cantly down-regulated,mainly annotated to secondary metabolites biosynthesis,bile secretion,and metabolism path-way.KEGG bubble chart analysis showed that phenylpropane biosynthesis,phenylalanine metabolism,and methane metabolism pathway had important effects on muscle growth during selection.Breeding exerted a significant effect on muscle metabolites in landlocked O.masou.Metabolomics analysis showed that post-selection,improvements in muscle nutritional quality relative to the non-selected group may be linked to metabolites changes such as DHA,EPA,AMPK,and PPARα in the phenylpropane biosynthesis and metabolism,fat metabolism,and arachidonic acid metabolism pathway.关键词
陆封型种群/选育群体/非靶向代谢组学/肌肉/马苏大麻哈鱼Key words
Landlocked population/Selected population/Untargeted metabolomics/Muscle/Oncorhynchus masou分类
农业科技引用本文复制引用
金笑延,韩明铭,刘慧吉,曲桂娟,牛小天,郝永坤,陈秀梅,王桂芹..基于代谢组学分析选育系F5代与非选育系马苏大麻哈鱼肌肉代谢物的差异[J].水生生物学报,2026,50(1):1-9,9.基金项目
国家现代农业产业技术体系(CARS-46) （CARS-46）
吉林省科技厅重点研发项目(20240303073NC) （20240303073NC）
吉林水产产业技术体系(JLARS-2025-130302)资助[Supported by the earmarked fund for China Agriculture Research System(CARS-46) （JLARS-2025-130302）
the Key R&D Project of the Science and Technology Department of Jilin Province(20240303073NC) （20240303073NC）
the earmarked fund for JLARS-Aquaculture(JLARS-2025-130302)] （JLARS-2025-130302）
