水生生物学报2026，Vol.50Issue(1)：71-82,12.DOI:10.3724/1000-3207.2025.2025.0130
饲料中添加不同来源的虾青素对虹鳟生长性能、肉色、抗氧化能力和抗急性亚硝酸盐胁迫的影响
DIFFERENT ASTAXANTHIN SOURCES ON GROWTH PERFORMANCE,FLESH COLOR,ANTIOXIDANT ABILITY,AND ACUTE NITRITE STRESS OF RAINBOW TROUT(ONCORHYNCHUS MYKISS)
摘要
Abstract
The present study investigated the effects of dietary supplementation with astaxanthin from different sources on growth performance,flesh pigmentation,antioxidant capacity,and acute nitrite stress response in rainbow trout(Oncorhynchus mykiss).Four diets were designed as the control diet and three astaxanthin inclusion diets,including 3.0 g/kg Paracoccus carotinifaciens(PC),2.4 g/kg Haematococcus pluvialis(HP),and 0.6 g/kg Carophyll Pink(CP),each providing 60 mg/kg astaxanthin.A total of 480 rainbow trout with an initial body weight of(6.17±0.08)g were randomly assigned to four groups with four replicates each,followed by a 30-day feeding trial.Results showed that there were no significant differences in growth performance between astaxanthin-supplemented groups and the control group(P>0.05).Muscle redness and yellowness values,as well as astaxanthin content,were significantly higher in all astaxanthin-supplemented groups than that in the control group(P<0.05),with the CP group showing the highest asta-xanthin deposition in muscle.Antioxidant capacity was enhanced in all astaxanthin-treated groups,as evidenced by significantly elevated hepatic activities of superoxide dismutase(SOD),total antioxidant capacity(T-AOC),and cata-lase(CAT),along with reduced malondialdehyde(MDA)levels before and after acute nitrite exposure(P<0.05).Following nitrite challenge,plasma cortisol,glucose,and lactate levels were significantly lower in astaxanthin-fed groups compared to the control(P<0.05).Furthermore,astaxanthin supplementation significantly upregulated hepatic nrf2 and sod gene expression and downregulated bcl2 and caspase9 expression.In conclusion,dietary astaxanthin from three different sources improved the flesh color,pigmentation,antioxidant capacity,and anti-stress capacities in rain-bow trout.However,PC and CP showed stronger effects in enhancing antioxidant capacity compared to HP,while HP exhibited a greater ability to alleviate cell apoptosis in rainbow trout.关键词
虾青素/肉色/抗氧化能力/亚硝酸盐胁迫/虹鳟Key words
Astaxanthin/Flesh color/Antioxidant capacity/Nitrite stress/Oncorhynchus mykiss分类
农业科技引用本文复制引用
邹羽羽,刘昊昆,陈鉴,金俊琰,韩冬,张志敏,朱晓鸣,杨云霞,解绶启..饲料中添加不同来源的虾青素对虹鳟生长性能、肉色、抗氧化能力和抗急性亚硝酸盐胁迫的影响[J].水生生物学报,2026,50(1):71-82,12.基金项目
国家重点研发计划(2023YFD2400600) （2023YFD2400600）
国家自然科学基金(32473179和32202958) （32473179和32202958）
水产品种创制与高效养殖全国重点实验室(2024BBSA09) （2024BBSA09）
湖南省重点研发计划(2024WK2015)资助[Supported by the National Key R&D Program of China(2023YFD2400600) （2024WK2015）
National Natural Science Foundation of China(32473179 and 32202958) （32473179 and 32202958）
the Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture(CAS)(2024BBSA09) （CAS）
Science and Technology Innovation Program of Hunan Province(2024WK2015)] （2024WK2015）
