水生生物学报2026，Vol.50Issue(1)：83-93,11.DOI:10.3724/1000-3207.2025.2025.0111
尖鳍鮈线粒体全基因组的测定及鮈亚科系统发育分析
THE COMPLETE MITOCHONDRIAL GENOME OF GOBIO ACUTIPINNATUS AND PHYLOGENETIC ANALYSIS OF GOBIONINAE SUBFAMILY
摘要
Abstract
Gobio acutipinnatus is a native and unique fish species in the Irtysh River Basin,as an important food source for carnivorous fish,it plays a crucial role in maintaining the biodiversity and ecological balance of the region.In order to understand the mitochondrial genome of G.acutipinnatus and clarify its phylogenetic position in the subfamily Gobioninae,we sequenced its complete mitochondrial genome and constructed a molecular phylogenetic tree of Gobioninae based on nucleotide sequences of 13 protein-coding genes.The results showed that the complete mitochon-drial genome of G.acutipinnatus was 16608 bp in length,consisting 13 protein-coding genes,2 rRNA genes,22 tRNA genes,1 non-coding region,and 1 origin of light-strand replication(OL)region.The base composition exhibits a signi-ficant AT preference(A+T=54.90%).Except for the protein-coding gene nad6 and 8 tRNA genes(trnQ,trnA,trnN,trnC,trnY,trnS2,trnE,and trnP)located outside the light chain,the remaining 28 genes were all located in the heavy chain.Among the 22 tRNA genes,only trnS1 gene lacked the DHU arm,while the other tRNAs could fold into the standard cloverleaf secondary structure,with a total of 57 G-U mismatches pairs.The phylogenetic tree(based on 13 protein-coding genes)revealed that the genus Saurogobio was embedded within Gobio,together with Mesogobio and Acanthogobio,they formed a branch.Gobio acutipinnatus clustered with Gobio occitaniae,Gobio cynocephalus,Gobio lozanoi,Gobio macrocephalus,Gobio soldatovi,and Gobio gobio within the genus Gobio.This study is the first to cla-rify the structural characteristics of the complete mitochondrial genome of G.acutipinnatus and determine its phyloge-netic position,providing key molecular evidence for understanding the taxonomic relationships of this species within the genus Gobio and the Gobioninae subfamily.关键词
线粒体基因组/基因测序/系统发育分析/尖鳍鮈Key words
Mitochondrial genome/Gene sequencing/Phylogenetic analysis/Gobio acutipinnatus分类
生物科学引用本文复制引用
吴冰倩,岳城,郝翠兰,张丽,夏深圳,杨圆圆,马玉兰,李志恒,柳方,杨钰盟,朱海林..尖鳍鮈线粒体全基因组的测定及鮈亚科系统发育分析[J].水生生物学报,2026,50(1):83-93,11.基金项目
国家自然科学基金(32403085) （32403085）
新疆维吾尔自治区重点研发计划(2023B02012) （2023B02012）
新疆农业大学大学生创新项目(dxscx2024446)资助[Supported by the National Natural Science Foundation of China(32403085) （dxscx2024446）
Key Research and Develop-ment Program for Special Project of Xinjiang(2023B02012) （2023B02012）
Xinjiang Agricultural University's Undergraduate Innovation Project(dxscx2024446)] （dxscx2024446）
