生物加工过程2025，Vol.23Issue(6)：639-648,10.DOI:10.3969/j.issn.1672-3678.2025.06.005
代谢工程在典型聚羟基脂肪酸酯(PHAs)合成中的应用
Metabolic engineering for the synthesis of typical polyhydroxyalkanoates
摘要
Abstract
Polyhydroxyalkanoates(PHAs),as a class of polyesters synthesized by microorganisms,can be processed into biodegradable plastics after purification.Metabolic engineering of microorganisms is widely utilized to produce various types of PHAs,serving as potential alternatives for traditional petroleum-based plastics such as polyethylene(PE),polypropylene(PP),and polyethylene terephthalate(PET).More importantly,the use of PHAs can reduce environmental pollution caused by non-degradable plastics.In this review,we outlined the typical PHAs products and summarized recent advances in related metabolic engineering strategies:controlling the expression of key enzymes through modulation of gene dosage(promoter engineering,ribosome binding site(RBS)optimization,etc.);modifying host strains using genome editing technologies such as CRISPR/Cas9;optimizing metabolic flux distribution based on cofactor engineering(NADH/NAD+balance);and enhancing the catalytic efficiency of PHA synthase(PhaC)through engineering.Additionally,we presented the next-generation industrial biotechnology(NGIB)platform,exemplified by the halophilic bacterium Halomonas bluephagenesis TD01.Due to its unique advantages in open,unsterile cultivation and high-yield production,this platform is driving innovative developments in PHAs manufacturing technology.关键词
PHAs/代谢工程/盐单胞菌TD01/下一代工业生物技术Key words
PHAs/metabolic engineering/Halomonas bluephagenesis TD01/next generation industrial biotechnology分类
生物科学引用本文复制引用
张宇航,熊依娜,王舒禾,凌晨..代谢工程在典型聚羟基脂肪酸酯(PHAs)合成中的应用[J].生物加工过程,2025,23(6):639-648,10.基金项目
国家自然科学基金(32500087) （32500087）
厦门市自然科学基金(3502Z202471027) （3502Z202471027）
