生物加工过程2025，Vol.23Issue(6)：660-669,10.DOI:10.3969/j.issn.1672-3678.2025.06.007
维生素C生物合成的微生物代谢工程改造研究进展
Research progress on metabolic engineering for microbial biosynthesis of vitamin C
摘要
Abstract
Vitamin C,as an essential water-soluble vitamin,is widely applied in pharmaceutical,food,and cosmetic industries,and its industrial production has long relied on microbial fermentation synthesis.Herein,we summarized recent advances in the biosynthesis of vitamin C,the optimization of fermentation process,and metabolic engineering of the corresponding precursor 2-keto-L-gulonic acid(2-KLG).By comparison of the two synthetic pathways,the traditional"two-step three-strain"fermentation and emerging one-step fermentation,we described their advantages and disadvantages with regards to the efficiency and cost.In addition,we highlighted several research cases of both mixed-culture and single-culture one-step fermentation.This review was focused on the metabolic-engineering strategies of chassis cells,including the genetic regulation of sorbitol,sorbose and sorbosone metabolism and the directed remodeling of dehydrogenases in Gluconobacter oxydans,Ketogulonicigenium vulgare and other microorganisms.It also covered advances in fermentation conditions,cofactor engineering and AI-assisted enzyme screening.Finally,based on the latest applications of scaffold proteins,microcompartment structures and gene-editing technologies,we presented future directions for one-step vitamin C production.关键词
维生素C/2-酮基-L-古龙酸(2-KLG)/代谢工程/一步法发酵/发酵工艺优化/生物合成Key words
vitamin C/2-KLG/metabolic engineering/one-step fermentation/fermentation process optimization/biosynthesis分类
生物科学引用本文复制引用
于丁一,彭银,崔吉晓,刘其敬,钟成..维生素C生物合成的微生物代谢工程改造研究进展[J].生物加工过程,2025,23(6):660-669,10.基金项目
山东省重点研发计划(重大科技创新工程)(2022CXGC010506) （重大科技创新工程）
