生物加工过程2025，Vol.23Issue(6)：682-691,10.DOI:10.3969/j.issn.1672-3678.2025.06.009
基于农用固体废弃物再利用的柠檬烯生物制备
Bio-production of limonene based on the utilization of agricultural solid waste
摘要
Abstract
The utilization of agricultural solid wastes,which represent abundant and low-cost carbon sources,has become a research focus in biomanufacturing with regards to their conversion to natural products by microbial cell factories.D-limonene,a monocyclic monoterpenoid compound,has demonstrated extensive applications in biopesticides,food additives,pharmaceutical intermediates,and cosmetic ingredients due to its characteristic double-bond structure and biological activities.Through pathway reconstruction and optimization,we previously developed an engineered Saccharomyces cerevisiae strain MKL8,leading to de novo D-limonene production.Herein,our continuous efforts resulted in further improvement of D-limonene production,from 452.65 mg/L to 878.08 mg/L,by the disruption of sterol transcriptional repressor MOT3 and overexpression of the activator UPC2-1(G888D).Subsequent screening showed that single knockout of YER134C,a regulatory gene in D-limonene biosynthesis,could significantly increase the production to 1 029.24 mg/L.Moreover,medium optimization was performed using crushed spoiled lemon solution as an auxiliary carbon source.As a result,the addition of 2%(V/V)such supplementation further improved the D-limonene production to 1 129.75 mg/L.When the crushed spoiled lemon solution was served as the sole nutrient source,the strain could afford the de novo synthesis of 20.25 mg/L D-limonene.Our research work demonstrated great value for the utilization of waste-derived resources in D-limonene production.关键词
D-柠檬烯/酿酒酵母/代谢工程/农用固体废弃物/资源化利用Key words
D-limonene/Saccharomyce scerevisiae/metabolic engineering/agricultural solid waste/resource utilization分类
生物科学引用本文复制引用
花若薇,徐显皓,刘延峰,李江华,堵国成,吕雪芹,刘龙..基于农用固体废弃物再利用的柠檬烯生物制备[J].生物加工过程,2025,23(6):682-691,10.基金项目
重庆市生态环境局科研项目(2023-003) （2023-003）
