山西大学学报（自然科学版）2026，Vol.49Issue(1)：179-188,10.DOI:10.13451/j.sxu.ns.2024078
磺胺对黑水虻幼虫生长及肠道微生物群落的影响
Effects of Sulfonamide on the Growth and Gut Microbial Communities of Black Soldier Fly Larvae
摘要
Abstract
Sulfonamides(SAs)antibiotics are one of the widely used antibiotics in livestock and poultry farming.They have high re-sidual levels in livestock manure and can enter the environment through feces,accelerating the formation of drug-resistant microor-ganisms and the spread of drug resistance,and posing a threat to human health.Black soldier fly larvae(BSFL)are important corro-sive insects,which can efficiently degrade livestock manure.It is of great significance to study the effects of sulfonamide in live-stock manure on the growth of black soldier fly larvae and composition of intestinal microbial community for black soldier fly larvae transforming into livestock manure containing antibiotic and reducing antibiotic pollution.Thus,This study exposed the black sol-dier fly larvae to pig manure containing 1 000 μg/kg sulfonamide.The composition of gut microbiota in larvae was analyzed using 16SrRNA high-throughput sequencing technology,and the effects of sulfonamide antibiotics on larval growth,waste reduction rate,and gut microbiota structure were explored.The results showed that the weight of larvae and waste reduction rate were significantly higher than those of the control group after the addition of sulfonamide.The results of dilution curve,Venn plot,and diversity index analysis showed that the diversity of gut microbiota in black soldier fly larvae were significantly reduced after sulfonamide addition treatment.Firmicutes,Bacteroidota,Proteobacteria,and Actinobacteriota were the main bacterial groups in the gut of black soldier fly larvae.The sulfonamide addition affected the composition of gut microbiota.The relative abundance of Clostridium sensu stricto 1 genus was significantly increased,while other dominant genera,for example,the relative abundance of the genera Terrisporobac-ter,Dysgonomonas,Enterococcus,and Turicibacter were decreased,while Fluviicola,Sedimentibacter,Sarcina,and Comamonas were only found in the control group.In addition,the results of the microbial co-currence network indicated that 1 000 μg/kg sulfon-amide reduces the complexity and stability of the gut microbiota of black soldier fly larvae.关键词
腐食性昆虫/抗生素/微生物多样性/猪粪/基质转化率Key words
corrosive insect/antibiotic/microbial diversity/pig manure/waste reduction分类
生物科学引用本文复制引用
郭治雪,刘爽,郝建伟..磺胺对黑水虻幼虫生长及肠道微生物群落的影响[J].山西大学学报（自然科学版）,2026,49(1):179-188,10.基金项目
山西省科技合作交流专项(202404041101050 （）
202104041101044) （）
2023年小店区山西大学产学研合作项目(202301S04) （202301S04）
