现代检验医学杂志2026，Vol.41Issue(1)：11-14,57,5.DOI:10.3969/j.issn.1671-7414.2026.01.003
莱菔素调控ANO1/LXR通路降低食管癌细胞胆固醇代谢并抑制侵袭迁移的实验研究
Experimental Study of Sulforaphene Regulation of the ANO1/LXR Pathway to Reduce Cholesterol Metabolism and Inhibit Invasion and Migration in Esophageal Cancer Cells
摘要
Abstract
Objective To investigate the effects of sulforaphene on invasion,metastasis and cholesterol metabolite levels of esopha-geal cancer cells,and its potential mechanisms through regulating the calcium-activated chloride channel protein 1(ANO1)/liver X receptor(LXR)pathway.Methods Human esophageal epithelial cells(HEEC)and esophageal cancer cell lines EC109,KYSE510,KYSE150,KYSE30 and TE-11 were cultured.Real-time quantitative PCR(RT-qPCR)was used to detect mRNA levels of ANO1 and LXR in the cells.EC109 cells were selected as the objects for subsequent studies.EC109 cells were treated with different con-centrations of sulforaphene,and cell viability was assessed using CCK-8 kit,and 20 μmol/L sulforaphene was selected as the con-centration for subsequent studies.EC109 cells were further cultured and divided into esophageal cancer control group,sulforaphene group and ANO1 overexpression group.The invasive and migration ability of EC109 cells was detected by Transwell assays and scratch assays.Protein levels of ANO1 and LXR in EC109 cells were detected by Western blotting.Cholesterol content in EC109 cells was detected by ELISA.The levels of metabolism-related proteins ATP-binding cassette transporter-1(ABCA1),ATP citrate lyase(ACLY)and peptidylprolyl isomerase B(PPIB)in EC109 cells were detected by immunofluorescence staining.Results Compared with HEEC cells,esophageal cancer cells EC109,KYSE510,KYSE150,KYSE30 and TE-11 cells exhibited increased ANO1 mRNA level and decreased LXR mRNA level,with statistically significant differences(F=90.77,59.76,all P<0.05).Among these cell lines,EC109 showed the most pronounced ANO1 and LXR expressions,making it the subject of subsequent studies.Treatment of EC109 cells with different concentrations of sulforaphene(0,5,10,20,40,80 μmol/L)for 48h rendered the progres-sive decrease of cell viability with increasing sulforaphene concentration(F=454.80,P<0.05).20 μmol/L of sulforaphene inhibited approximately half of the cell viability,thus this concentration was selected for subsequent experiments.Compared with esophageal cancer control group,sulforaphene group exhibited reduced invasion and migration capacity,decreased ANO1 protein expression,increased LXR protein expression,and reduced cholesterol content along with decreased expression of cholesterol metabolism-re-lated proteins ABCA1,ACLY and PPIB(t=7.22～17.70,all P<0.05).Compared with sulforaphene group,the ANO1-overexpressing group exhibited increased ANO1 protein expression,decreased LXR protein expression(t=10.61,8.48,P<0.001),increased choles-terol content,and elevated expression of cholesterol metabolism-related proteins ABCA1,ACLY,PPIB(t=6.11～10.87,all P<0.05).Conclusions Sulforaphene can regulate the ANO1/LXR pathway to inhibit the invasion and metastasis of esophageal cancer cells,which may be related to its regulation of cholesterol metabolite levels.关键词
食管癌/莱菔素/钙激活氯通道蛋白1(ANO1)/肝X受体(LXR)通路/胆固醇代谢物/侵袭/迁移Key words
esophageal cancer/sulforaphene/ANO1/LXR pathway/cholesterol metabolites/invade/migration分类
医药卫生引用本文复制引用
金秋,刘永芳,李志国,范花平,孙鹏博,程丽敏..莱菔素调控ANO1/LXR通路降低食管癌细胞胆固醇代谢并抑制侵袭迁移的实验研究[J].现代检验医学杂志,2026,41(1):11-14,57,5.基金项目
河北省卫健委2023年度医学科学研究课题(20231946). （20231946）
