现代检验医学杂志2026，Vol.41Issue(1)：24-28,5.DOI:10.3969/j.issn.1671-7414.2026.01.006
M1型巨噬细胞外泌体通过JAK1/STAT3信号通路抑制卵巢癌细胞免疫逃逸
Study on the Inhibition of Ovarian Cancer Cell Immune Escape by M1 Macrophage Exosomes through JAK1/STAT3 Signaling Pathway
摘要
Abstract
Objective To investigate the influence of M1 macrophage-derived exosomes on the JAK1/STAT3 pathway and its impact on ovarian cancer cell behavior.Methods Isolated extracellular vesicles(M1 exo)from M1 macrophages and incubated them with human ovarian cancer SKOV3 cells at 0,50 and 100 μg/ml for 48 hours,labeled as PBS,M1 exo(50 μg/ml)and M1 exo(100 μg/ml)groups.The plate cloning assay was employed to assess SKOV3 cell proliferation,and the Transwell assay for evaluating SKOV3 cell migration and invasion.Flow cytometry was used to measure SKOV3 cell apoptosis,and Western blotting for analyzing p-JAK1 and p-STAT3 protein expression.Additionally,NK-92MI natural killer cells were co-cultured with the aforementioned groups,and the supernatant's IL-6 and TNF-α levels,as well as the immune killing rate of NK-92MI in the co-culture system,were assessed.Results The isolated M1 exo exhibited the typical morphology of extracellular vesicles and strongly expressed marker proteins CD9,CD81 and TSG101.PKH67 staining indicatesd that M1 exo was internalized by SKOV3 cells.The 50 μg/ml M1 exo groups showed a significant reduction in SKOV3 cell migration,invasion and apoptosis,as well as decreased p-JAK1 and p-STAT3 protein expression,compared to the PBS group(t=4.08～21.63,P<0.05).The 100 μg/ml M1 exo group further suppressed SKOV3 cell proliferation,migration,invasion and apoptosis,and lowers p-JAK1 and p-STAT3 expression,compared to the 50 μg/ml group(t=3.61～15.60,P<0.05).Following co-culture,the supernatant of both M1 exo groups demonstrated a marked increase in IL-6 and TNF-α levels and the immune killing rate of NK-92MI cells,compared to the PBS group(t=21.76～14.88,P<0.05).Notably,the 100 μg/ml M1 exo group exhibited the highest levels of IL-6,TNF-α and NK-92MI immune killing rate,with statistically significant differences(t=4.41～6.88,P<0.05).Conclusions M1 macrophage ex-tracellular vesicles may inhibit SKOV3 cell proliferation,migration,invasion,and immune escape by downregulating the JAK1/STAT3 signaling pathway,promoting cell apoptosis.关键词
卵巢癌/M1型巨噬细胞/外泌体/免疫逃逸/酪氨酸激酶1/信号转导和转录激活因子3通路Key words
ovarian cancer/M1 type macrophages/extracellular vesicles/immune escape/JAK1/STAT3 pathway分类
医药卫生引用本文复制引用
陈淑贤,钱红霞,吕晓芳..M1型巨噬细胞外泌体通过JAK1/STAT3信号通路抑制卵巢癌细胞免疫逃逸[J].现代检验医学杂志,2026,41(1):24-28,5.基金项目
河北省卫健委2024年度医学科学研究课题(编号:20242378). （编号:20242378）
