现代检验医学杂志2026，Vol.41Issue(1)：29-34,6.DOI:10.3969/j.issn.1671-7414.2026.01.007
肿瘤细胞来源外泌体miR-21诱导巨噬细胞焦亡促进胃癌细胞转移机制的实验研究
Experimental Study on the Mechanism of Tumor Cell-Derived Exosomes miR-21 Inducing Macrophage Pyroptosis to Promote Metastasis in Gastric Cancer Cells
摘要
Abstract
Objective To explore the effect of tumor cell-derived exosomal miR-21 on macrophage pyroptosis during gastric can-cer cell metastasis.Methods Human gastric cancer cell line HGC-27 and human normal gastric epithelial cell GES-1 were cul-tured and exosomes were extracted.The expression of miR-21 in exosomes was detected by qRT-PCR.HGC-27 cell line stably over expressing miR-21 and negative control cell line were constructed.Exosomes were extracted and labeled with PKH67 dye.The exosomes were divided into miR-21-exo group,miR-21-mimics-exo group and miR-21-mimicsNC-exo group.THP-1 cells were induced to differentiate into M0 macrophages using PMA and grouped into exo-miR-21,exo-miR-21-mimics,exo-miR-21-mimicsNC.After 36 hours of treatment with exosomes,the expression levels of macrophage scorch-related factors were detected by Western blot and ELISA.The supernatants were extracted and co-cultured with HGC-27 cells.The migration and invasion of HGC-27 cells were detected by scratch test and Transwell test.Results MiR-21 was highly expressed in HGC-27 cell-derived exosomes compared with GES-1 cell-derived exosomes,and the difference was statistically significant(t=12.00,P<0.001).Com-pared with the exo-miR-21 group and the exo-miR-21-mimicsNC group,the expression level of macrophage pyroptosis-related factors in the exo-miR-21-mimics group was significantly higher,the differences were statistically significant(t=19.23～26.79,all P<0.000 1).Compared with HGC-27 cells added with macrophage culture supernatants from the exo-miR-21 intervention group and the exo-miR-21-mimicsNC intervention group,HGC-27 cells with the addition of exo-miR-21-mimics had significantly en-hanced migration and invasion ability in the supernatant of macrophage culture,the differences were statistically significant(tmigra-tion=11.39,11.16,tinvasion=10.80,9.153,all P<0.001).Conclusions Tumor cell-derived exosomal miR-21 are able to promote gastric cancer cell metastasis by inducing macrophage pyroptosis.关键词
外泌体/微小RNA-21/巨噬细胞/细胞焦亡/胃癌Key words
exosomes/miR-21/macrophages/pyroptosis/gastric cancer分类
医药卫生引用本文复制引用
张伟,蔡振花,杨晨,李浩闯,魏琦,王玉宏..肿瘤细胞来源外泌体miR-21诱导巨噬细胞焦亡促进胃癌细胞转移机制的实验研究[J].现代检验医学杂志,2026,41(1):29-34,6.基金项目
河北省卫生健康委2022年度医学课题(20220017). （20220017）
