天津科技大学学报2025，Vol.40Issue(6)：18-28,11.DOI:10.13364/j.issn.1672-6510.20240033
后生元牙膏及牙膏中的SLS对口腔生物膜抗龋性的影响
Effects of Postbiotic Toothpaste and SLS in Toothpaste on the Caries Resistance of Oral Biofilm
摘要
Abstract
In this study,an in vitro model of the oral microbiome was developed to investigate the effects of postbiotic toothpaste(PT)and sodium dodecyl sulfate(SLS)in toothpaste on the caries resistance of oral biofilms,using crystal violet staining,total colony-forming unit(CFU)counting,polymerase chain reaction(PCR)analysis,lactic acid concentration de-termination and 16S rDNA sequencing.The results showed that PT maintained the normal growth and metabolism of healthy biofilms,and after cariogenic challenge,the PT group exhibited lower viable living bacteria counts,total bacterial load,and metabolic activity,indicating enhanced caries resistance.In contrast,SLS disrupted the growth,metabolism,and three-dimensional structure of healthy biofilms,and after cariogenic challenge,the SLS group showed increased biomass,viable bacterial counts,total bacterial load,and metabolic activity,leading to a higher risk of caries development.Biofilm commu-nity analysis revealed that the PT group exhibited α-diversity and community structure similar to those of the negative control group,and remained relatively stable after cariogenic challenge.By contrast,the SLS group displayed high α-diversity,but its flora structure was dysregulated and unstable.The results of the combined PT and SLS intervention group showed that the SLS content in the postbiotic toothpaste was insufficient to cause oral microecological dysregulation,as PT was effective in counteracting the detrimental effects of SLS on biofilm.This study may provide a reference for the development of effective ingredients for oral care products.关键词
后生元牙膏/十二烷基硫酸钠/口腔微生态/生物膜/龋病Key words
postbiotic toothpaste/SLS/oral microecology/biofilm/dental caries分类
医药卫生引用本文复制引用
高静,孙莲莲,师庆英,黄正梅,路福平,赵化冰..后生元牙膏及牙膏中的SLS对口腔生物膜抗龋性的影响[J].天津科技大学学报,2025,40(6):18-28,11.基金项目
中国博士后科学基金面上资助项目(2023M732614) （2023M732614）
