天津科技大学学报2025，Vol.40Issue(6)：29-36,75,9.DOI:10.13364/j.issn.1672-6510.20240123
大肠杆菌利用甘露糖生产2'-岩藻糖基乳糖的途径构建及优化
Construction and Optimization of the Pathway from Mannose to 2'-Fucosyllactose in Escherichia coli
摘要
Abstract
2'-fucosyllactose(2'-FL)is a fucosyl-modified human milk oligosaccharides,which plays an important role in infant growth.2'-FL could be synthesized by microorganism with high production;however,the low carbon source utiliza-tion efficiency in this method limits the continuous improvement of 2'-FL production.In this study,a new strategy was de-veloped to enable the strain to utilize glycerol for its self-growth and mannose for the production of 2'-FL,with cell growth and production not interfering with each other.The phosphomannose isomerase gene(manA)in Escherichia coli strain was deleted,allowing the strain to use glycerol for cell growth and mannose for 2'-FL synthesis.This modification significantly improved the 2'-FL conversion rate from 10.77%to 53.80%.Furthermore,by knocking out the branching pathway of the precursor GDP-fucose and optimizing the expression levels of key enzymes α-1,2-fucosyltransferase and mannose-1-phosphate guanyloyltransferase,the production of 2'-FL was further increased.The effectiveness of this strategy was verified by measuring strain growth and mannose conversion rate.This method will be applied to existing 2'-FL high-producers in the laboratory to further improve the conversion rate of mannose to 2'-FL,thereby providing a reference for the construction of industrial strains with efficient synthesis of 2'-FL.关键词
2'-岩藻糖基乳糖/人乳寡糖/甘露糖/大肠杆菌/微生物合成Key words
2'-fucosyllactose/human milk oligosaccharides/mannose/Escherichia coli/microbial synthesis分类
化学化工引用本文复制引用
李莎,胡雅雯,李俊众,孙雪,李玉,李庆刚..大肠杆菌利用甘露糖生产2'-岩藻糖基乳糖的途径构建及优化[J].天津科技大学学报,2025,40(6):29-36,75,9.基金项目
国家重点研发计划项目(2022YFC2104902) （2022YFC2104902）
天津市研究生科研创新项目(2022BKY134) （2022BKY134）
