西北林学院学报2026，Vol.41Issue(1)：93-106,118,15.DOI:10.3969/j.issn.1001-7461.2026.01.09
杨树TCP基因家族生物信息学与盐胁迫应答表达分析
Bioinformatics Analysis of Poplar TCP Gene Family and Its Response to Salt Stress
摘要
Abstract
TCPs are plant-specific transcription factors.This study aims to investigate the structural charac-teristics of the poplar TCP gene family and its expression properties under salt stress,with a view to provi-ding more ideas for the selection and breeding of superior genes in poplar.The physicochemical properties,gene structures,cis-acting elements,protein interactions,and intra-and inter-species covariance of the pop-lar TCP gene family were analyzed by means of bioinformatics.The expression patterns under high salt stress were analyzed by RNA-seq data.A total of 37 non-redundant TCP gene family members were identified in poplar,of which 20 belonged to the Class Ⅰ subfamily and 17 to the Class Ⅱ subfamily(CYC and CIN subclasses contain 7 and 10 genes,respectively).The structure,conserved motif distribution,con-served structural domains,and protein tertiary structures of TCP genes of the same subfamily were highly similar.The promoter sequences of TCP genes contained various hormone response elements,stress re-sponse elements,and developmental process-specific elements and et al.Analysis of the interacting-proteins showed that there were 26 key hub proteins among the 37 TCP members,among which Potri.008G115800.1,Potri.003G120200.1 and Potri.001G111800.1 played central regulatory roles in the cell.Collinearity analysis showed that there were 8 pairs of covariance relationships within the species,and the two genes with collinearity with each other had similar gene sequences on different chromosomes.Analysis of the transcriptome data revealed that there were 6,1,and 7 TCPs genes were significantly differentially ex-pressed in poplar leaf,stem,and root after salt stress treatment,respectively,and verified by RT-qPCR.This study systematically analyzed the structural characteristics and expression properties of the poplar TCP gene family from various aspects,and provided a reference basis for the future research of the regula-tory mechanism of TCP transcription factors in poplar under high salt stress.关键词
杨树/TCP/生物信息学/盐胁迫Key words
Populus trichocarpa/TCP/bioinformatics/salt stress分类
农业科技引用本文复制引用
刘德麒,何家劲,程紫涵,张雪梅..杨树TCP基因家族生物信息学与盐胁迫应答表达分析[J].西北林学院学报,2026,41(1):93-106,118,15.基金项目
湖北省自然科学基金项目(2023AFB298). （2023AFB298）
