西南农业学报2025，Vol.38Issue(12)：2619-2631,13.DOI:10.16213/j.cnki.scjas.2025.12.009
油茶β-酮脂酰-ACP合成酶Ⅲ基因家族鉴定及表达特性分析
Identification and expression characteristics of β-ketoacyl-ACP synthase Ⅲ gene family in Camellia oleifera
摘要
Abstract
[Objective]β-ketoacyl-ACP synthase Ⅲ(KAS Ⅲ),as the core initiating enzyme in the plant fatty acid synthesis pathway,plays a key role in regulating oil accumulation and fatty acid synthesis.By analyzing the expression patterns and functional characteristics of the Co-KAS Ⅲ gene family in different tissues and under various stress conditions in Camellia oleifera,the present study aims to provide a theoretical foundation for further elucidating the biological functions of CoKAS Ⅲ genes.[Method]Members of the CoKAS Ⅲ gene family in hexaploid C.oleifera were systematically identified using bioinformatics methods,followed by comprehensive analyses of their gene structures,evolution-ary relationships and expression profiles.[Result]A total of 15 CoKAS Ⅲ gene family members were identified,all containing the PLN02326 conserved domain specific to the KAS Ⅲ family.Members within the same subfamily exhibited highly consistent gene structures and function-al domains,suggesting functional similarity.Physicochemical property analysis revealed significant diversity among family members,and sub-cellular localization predictions indicated that these proteins were primarily distributed in the chloroplast(3),mitochondria(4),plasma membrane(5),organelle membrane(1)and extracellular space(1).Phylogenetic analysis classified CoKAS Ⅲ into four subfamilies,with subfamilies Ⅰ and Ⅱ clustering with homologous proteins from other plants,demonstrating evolutionary conservation,while subfamilies Ⅲ andⅣ were unique to hexaploid C.oleifera,indicating gene family expansion driven by polyploidization.Collinearity analysis elucidated the genet-ic relationships among C.oleifera,Arabidopsis thaliana,C.sinensis and C.vietnamensis.Promoter analysis identified numerous cis-acting ele-ments related to light response,hormonal regulation and stress response.Expression pattern analysis showed that A2.8G267000,C2.7G18850 and C2.13G163200 were specifically highly expressed in flower buds,while most genes were active in the early stages of seed development.C2.13G107700 was significantly expressed in both susceptible(SH)and resistant(RH)varieties.C1.13G106400 was markedly up-regula-ted in response to low-temperature stress(LS04,2 ℃).B2.11G177300 and B1.11G165900 were specifically expressed under short-day(SD)and long-day(LD)conditions,respectively,whereas B1.5G165800,B2.1G358800 and C2.7G188500 were specifically expressed un-der medium-day(MD)conditions.During seed germination,expression levels exhibited fluctuating changes.[Conclusion]The study identi-fies 15 CoKAS Ⅲ gene family members,which exhibit both evolutionary conservation and specificity.Differential expression patterns across tissues,anthracnose resistance,low-temperature stress and photoperiod responses suggest that this gene family plays important roles in the growth and development,disease resistance and adaptation to abiotic stresses in C.oleifera.关键词
油茶/β-酮脂酰-ACP合成酶Ⅲ(KAS Ⅲ)/基因家族/表达分析Key words
Camellia oleifera/β-ketoacyl-ACP synthase Ⅲ(KAS Ⅲ)/Gene family/Expression analysis分类
农业科技引用本文复制引用
成新琪,刘宏涛,刘文超,李文莹,程计华,占红亮,程军勇,朱华国..油茶β-酮脂酰-ACP合成酶Ⅲ基因家族鉴定及表达特性分析[J].西南农业学报,2025,38(12):2619-2631,13.基金项目
湖北省自然科学基金联合基金项目(2025AFD333) （2025AFD333）
湖北省高等学校优秀中青年科技创新团队(T2023025) （T2023025）
湖北省中央引导地方科技发展专项(2024CSA071) （2024CSA071）
