西南农业学报2025，Vol.38Issue(12)：2632-2643,12.DOI:10.16213/j.cnki.scjas.2025.12.010
木油桐MIKCC型MADS-box家族鉴定及其生殖器官发育优异单倍型挖掘
Genome-wide identification of MIKCC-type MADS-box genes in Vernicia montana and exploration of superior haplotypes regulating reproductive organ development
摘要
Abstract
[Objective]The present paper aimed to identify MIKCC-type MADS-box genes in Vernicia montana and explore superior haplo-types involved in the reproductive organ development regulatory network.[Method]Bioinformatics methods were used to analyze sequence characteristics,evolutionary relationships,conserved domains,chromosome localization,promoter elements and gene expression profiles of MIKCC-type MADS-box gene haplotypes at the whole-genome scale in V.montana.A yeast two-hybrid assay was conducted to study protein functional differentiation.[Result]A total of 31 MIKCC-type MADS-box genes were identified,among which 5 genes had amino acid se-quence haplotype differences,and 13 genes had cis-acting element haplotype differences.Analysis of promoter elements and gene expression suggested that MIKCC-type MADS-box genes in V.montana may broadly participate in floral organ development,floral transition and seed de-velopment through hormonal regulatory pathways.Among these genes,VmMADS3.1,VmMADS32.2 and VmMADS48.2 were identified as su-perior haplotypes.VmMADS3.1 may be associated with the delayed developmental timing of female flower formation in V.montana;Vm-MADS32.2 likely promoted stamen differentiation while suppressing carpel development through a dosage-dependent regulatory mechanism;whereas VmMADS48.2 may promote abortion of stamens in female flowers,and play critical roles in regulating ovule formation,seed size and morphological patterning.[Conclusion]VmMADS3.1,VmMADS32.2 and VmMADS48.2 were identified as superior haplotypes involved in the reproductive organ development regulatory network in V.montana.The study provides theoretical foundation for future application of MIKCC-type MADS-box genes in regulating sex differentiation and improving seed traits in V.montana.关键词
木油桐/MADS-box基因家族/MIKCC型/生殖器官发育/优异单倍型Key words
Vernicia montana/MADS-box gene family/MIKCC-type/Reproductive organ development/Superior haplotype分类
农业科技引用本文复制引用
董翔,刘雨欣,朱华国,李欣悦,李文莹..木油桐MIKCC型MADS-box家族鉴定及其生殖器官发育优异单倍型挖掘[J].西南农业学报,2025,38(12):2632-2643,12.基金项目
国家自然科学基金青年科学基金项目(32401637) （32401637）
湖北省自然科学基金青年项目(2024AFB006) （2024AFB006）
湖北省高等学校优秀中青年科技创新团队(T2023025) （T2023025）
黄冈师范学院博士启动基金项目(2024068) （2024068）
