中草药2026，Vol.57Issue(2)：393-399,7.DOI:10.7501/j.issn.0253-2670.2026.02.001
黄芪茎叶中2个新的降环阿尔廷烷型三萜类化合物
Two new trinorcycloartane triterpenoids from stems and leaves of Astragalus membranaceus
摘要
Abstract
Objective To investigate trinorcycloartane triterpenoids from the stems and leaves of Astragalus membranaceus(AMSL).Methods Comprehensive separation and purification were performed using silica gel,ODS column chromatography,and high-performance liquid chromatography(HPLC).The structures of the compounds were identified by modern spectroscopic techniques.The cytotoxic activities of the compounds against PC12 cells were assessed using the MTT assay.Results Two trinorcycloartane triterpenoids were isolated from the n-butanol fraction of the 70%ethanol extract of AMSL,namely 1α,3β,12β,16β-tetrahydroxy-25,26,27-trinorcycloartan-24-oic acid methyl ester(1)and 1α,3β,12β-trihydroxy-25,26,27-trinorcycloartan-24,16β-olide(2).The MTT assay showed that compounds 1 and 2 exhibited varying degrees of cytotoxicity against PC12 cells within the concentration range of 5-100 μmol/L.Conclusions Compounds 1 and 2 are identified as new trinorcycloartane triterpenoids,named cyclastraine K(1)and cyclastraine L(2),respectively.Both compounds 1 and 2 demonstrate cytotoxicity against PC12 cells.关键词
蒙古黄芪/三萜/降环阿尔廷烷型/大鼠嗜铬细胞瘤细胞系/黄芪茎叶环阿尔廷烷三萜K/黄芪茎叶环阿尔廷烷三萜LKey words
Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao/triterpenoids/trinorcycloartane/PC12 cells/cyclastraine K/cyclastraine L分类
医药卫生引用本文复制引用
程艳刚,郝祥榕,赵俪瑾,李佩,赵佳欣,张翼,王颖莉,谭金燕..黄芪茎叶中2个新的降环阿尔廷烷型三萜类化合物[J].中草药,2026,57(2):393-399,7.基金项目
山西中医药大学杏林英才计划(2025XK17) （2025XK17）
2025年度中医药产业发展现代化支撑项目(2025zyycy09) （2025zyycy09）
山西省中医药管理局中医药创新团队建设计划(zyytd2024033) （zyytd2024033）
山西中医药大学科技创新能力培育计划项目(2021PY-JC-09) （2021PY-JC-09）
山西中医药大学学科建设项目(2025XK35) （2025XK35）
