中草药2026，Vol.57Issue(2)：400-404,5.DOI:10.7501/j.issn.0253-2670.2026.02.002
隔山牛皮消中2个新化学成分研究
Two new chemical constituents from Cynanchum wilfordii
摘要
Abstract
Objective To investigate the chemical constituents and their anti-inflammatory activity of Cynanchum wilfordii.Methods Various chromatographic techniques including silica gel,ODS,and Sephadex LH-20 column chromatography were employed for the isolation and purification of compounds.Their structures were elucidated through comprehensive spectroscopic analysis including HR-ESI-MS,ESI-MS,1H-NMR,13C-NMR,HSQC,and HMBC.The anti-inflammatory activity of the compounds was evaluated using the Griess method.Results Two compounds were isolated and identified as(7'R,8'S)-dehydrodiconiferyl alcohol 9'-O-β-D-xylopyranoside(1)and 3β-[(O-β-D-glucopyranosyl)-oxy]-8β,14β-trihydroxycarda-5,20-(22)-dienolide(2).Compounds 1 and 2 inhibited NO production by 55.16%and 15.34%,respectively,with a median inhibition concentration(IC50)of 41.37 μmol/L for compound 1.Conclusion Compounds 1 and 2 are both new compounds,belonging to lignan and cardenolide types,respectively.They have been assigned the names cynawilforin A(1)and wilforcardenoside A(2).In vitro anti-inflammatory assays indicated that compound 1 exhibits certain anti-inflammatory activity.关键词
隔山牛皮消/木脂素/强心苷/抗炎活性/隔山牛皮消素A/隔山牛皮消强心苷AKey words
Cynanchum wilfordii(Maxim.)Hemsl/lignan/cardenolide/anti-inflammatory activity/cynawilforin A/wilforcardenoside A分类
医药卫生引用本文复制引用
张露元,周晶,娄红波,普开亮,李云璐,陈超,陈英飞,徐笑宇,沈勇..隔山牛皮消中2个新化学成分研究[J].中草药,2026,57(2):400-404,5.基金项目
云南省高校服务重点产业科技项目(FWCY-QYCT2024014) （FWCY-QYCT2024014）
建设面向南亚东南亚科技创新中心专项项目(202403AP140013) （202403AP140013）
