中国耳鼻咽喉颅底外科杂志2025，Vol.31Issue(6)：24-28,5.DOI:10.11798/j.issn.1007-1520.202525342
白细胞介素-4/髓系细胞触发受体-2信号通路在小鼠外耳道胆脂瘤组织的表达及意义
Expression and significance of the IL-4/TREM-2 signaling pathway in cholesteatoma tissues of the mouse external auditory canal
摘要
Abstract
Objective To investigate the expression and significance of interleukin-4(IL-4)/triggering receptor expressed on myeloid cells-2(TREM-2)signaling pathway-related factors in the tissues of mouse external auditory canal cholesteatoma(EACC).Methods Fifteen C57BL6/J mice were randomly assigned to an experimental group(n=11)and a control group(n=4).EACC model was established in the experimental group via external auditory canal ligation.Three months after the surgery,the external auditory canal tissues were collected from the mice of the experimental and the control group for real-time quantitative polymerase chain reaction,Western blot and enzyme-linked immunosorbent assay to detect the mRNA and protein expression levels of IL-4,TREM-2,IL-6,IL-8,IL-1β and tumor necrosis factor-α(TNF-α).Results The modeling success rate in the experimental group 3 months after surgery was 91％(10/11).Compared with the control group,the mRNA and protein expression levels of TREM-2,IL-6,IL-8,IL-1β,and TNF-αin the EACC tissues of the experimental group were significantly increased(P＜0.05),while the expression level of IL-4 was markedly reduced(P＜0.05).Conclusion The IL-4/TREM-2 signaling pathway and the inflammatory response it mediates may be involved in the occurrence and development of EACC in mice.关键词
外耳道胆脂瘤/IL-4/TREM-2/炎症因子/动物模型Key words
External auditory canal cholesteatoma/IL-4/TREM-2/Inflammatory factors/Animal model分类
医药卫生引用本文复制引用
郭秀琴,郑杨帆,卢雯娴,邹亚纯,郑佳怡,何观文..白细胞介素-4/髓系细胞触发受体-2信号通路在小鼠外耳道胆脂瘤组织的表达及意义[J].中国耳鼻咽喉颅底外科杂志,2025,31(6):24-28,5.基金项目
福建省自然基金面上项目(2023J011079) （2023J011079）
宁德师范学院医校融合发展专项重点项目(2024ZX71). （2024ZX71）
