中国耳鼻咽喉颅底外科杂志2025，Vol.31Issue(6)：53-59,7.DOI:10.11798/j.issn.1007-1520.202524496
AGR2通过调控TGF-β/SMAD信号通路对鼻咽癌细胞迁移和侵袭的影响
The effect of AGR2 on the migration and invasion of nasopharyngeal carcinoma cells by regulating the TGF-β/SMAD signaling pathway
摘要
Abstract
Objective To investigate the effect of anterior gradient 2(AGR2)on the migration and invasion of nasopharyngeal carcinoma(NPC)cells by regulating the transforming growth factor-β(TGF-β)/SMAD signaling pathway.Methods Human normal nasopharyngeal epithelial cells(NP69)and NPC cells(5-8F,6-10B,CNE-1)were cultured in vitro,and real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression of AGR2 mRNA in the cells.The 5-8F cells were randomly assigned into control group,sh-NC group,sh-AGR2 group,and sh-AGR2+SRI-011381 group(sh-AGR2+TGF-β signaling pathway activator SRI-011381).qRT-PCR and Western blot were applied to detect the transfection efficiency of AGR2.Cell proliferation was measured by CCK8 assay and colony formation assay.Cell migration was measured by scratch test.Transwell assay was used to measure cell invasion.Cell apoptosis was determined by flow cytometry.The protein expressions of PCNA,MMP-2,MMP-9,TGF-β1 and p-SMAD2/SMAD2 were detected by Western blot.Results Compared with NP69 cells,the AGR2 mRNA expression was elevated in NPC cells.Compared with the control group or sh-NC group,the OD450,clone number,scratch healing rate,invasion number,expression levels of PCNA,MMP-2,MMP-9,TGF-β1,and p-SMAD2/SMAD2 in the sh-AGR2 group decreased,and the apoptosis rate was increased(P＜0.05).Compared with the sh-AGR2 group,the OD450,clone number,scratch healing rate,invasion number,expression levels of PCNA,MMP-2,MMP-9,TGF-β1,and p-SMAD2/SMAD2 in the sh-AGR2+SRI-011381 group increased,and the apoptosis rate was decreased(P＜0.05).Conclusion The expression level of AGR2 is elevated in NPC cells,which in turn promotes the proliferation,migration and invasion abilities of NPC cells and inhibits cell apoptosis.This may be achieved by regulating the TGF-β/SMAD signaling pathway.关键词
鼻咽癌/前梯度蛋白2/转化生长因子-β/SMAD信号通路/细胞迁移/细胞侵袭Key words
Nasopharyngeal carcinoma/Anterior gradient 2/Transforming growth factor-β/SMAD signaling pathway/Cell migration/Cell invasion分类
医药卫生引用本文复制引用
金辉,黄文霞,王珍,吴龙军,梁耕田..AGR2通过调控TGF-β/SMAD信号通路对鼻咽癌细胞迁移和侵袭的影响[J].中国耳鼻咽喉颅底外科杂志,2025,31(6):53-59,7.基金项目
武汉市卫健委科研项目(WX23Z04). （WX23Z04）
