中国南方果树2025，Vol.54Issue(6)：40-44,5.DOI:10.13938/j.issn.1007-1431.20240460
化橘红药材来源植物化州柚组织培养技术研究
Study of tissue culture technology for Citrus grandis 'Tomentosa',the plant source of H uajuhong
摘要
Abstract
Huajuhong is a traditional Chinese medicine.and Citrus grandis'Tomentosa'is one of its plant sources.To establish a complete tissue culture technology system for C.grandis'Tomentosa',young stem segments of C.grandis Tomentosa'were used as explants to investigate the effects of different influencing factors on bud induction,proliferation,seedling strengthening,rooting culture as well as transplant survival.The results showed that the optimal disinfection method for stem segment explants was a 30-second immersion in 75％ethanol,followed by sterilization with 0.1％mercuric chloride(HgCl2)for 6-7 minutes.For primary culture,the optimal culture medium for axillary bud induction was MS+1.5 mg/L 6-BA+1.0 mg/L KT+0.5 mg/L NAA.For adventitious bud proliferation subculture,a suitable medium was MS+1.0 mg/L 6-BA+0.5 mg/L NAA.The optimal medium for seedling strengthening and rooting was 1/2 MS+1.0 mg/L IBA+0.5 mg/L NAA+1 g/L activated carbon.The best transplant substrate for tissue-cultured rooted seedlings was a mix of peat soil∶sheep manure soil∶perlite at a volume ratio of 2∶1∶1,which resulted in robust roots and a survival rate of over 75％.The established rapid propagation system through stem segment tissue culture of C.grandis'Tomentosa'can provide technical reference for large-scale seedling production and lay a theoretical foundation for its genetic transformation research.关键词
化橘红/化州柚/组织培养/增殖/繁殖系数Key words
Huajuhong/Citrus grandis 'Tomentosa'/tissue culture/proliferation/propagation coefficient引用本文复制引用
陈乾平,张占江,肖潇,李林轩,韦莹,潘丽梅..化橘红药材来源植物化州柚组织培养技术研究[J].中国南方果树,2025,54(6):40-44,5.基金项目
广西重点研发计划项目(桂科AB20159014) （桂科AB20159014）
广西中医药适宜技术开发与推广项目(GZSY2024002) （GZSY2024002）
广西中医药管理局项目(GXZYYZZ-202201) （GXZYYZZ-202201）
广西岐黄学者培养项目(GXQH202402)资助. （GXQH202402）
