中国饲料Issue(1)：30-35,6.DOI:10.15906/j.cnki.cn11-2975/s.2024110079-12
羊驼源枯草芽孢杆菌分离培养与鉴定
Isolation,cultivation,and identification of Bacillus subtilis originating from alpaca
摘要
Abstract
In order to obtain Bacillus subtilis strains with high stability,strong stress resistance,high protease activity,high amylase activity,and low drug resistance,three strains of Bacillus subtilis were isolated and purified from the fresh feces of healthy alpacas in this study.Subsequently,morphological identification,16S rRNA sequencing,protease tests,amylase tests,tolerance tests,and drug sensitivity tests were conducted to determine the species and functions of the strains.The results showed that the three selected strains were Gram-positive purple rods and were identified as Bacillus subtilis through 16S rRNA sequencing and sequence alignment,with the strain numbers 21K,25K,and 29K,respectively.Further experiments indicated that 29K exhibited the best overall tolerance characteristics among the three strains,showing high protease activity,high amylase activity,and low drug resistance.In summary,29K had high potential as a probiotic preparation of Bacillus subtilis.关键词
羊驼/枯草芽孢杆菌/分离/鉴定Key words
alpaca/Bacillus subtilis/isolation/identification分类
农业科技引用本文复制引用
李松达,余琦,李明,李欣南,徐丽佳,张恒川,许楚媛,李松励..羊驼源枯草芽孢杆菌分离培养与鉴定[J].中国饲料,2026,(1):30-35,6.基金项目
基于AI干预的幼龄反刍动物抗腹泻饲料关键技术研发与示范(2023-MSLH-090) （2023-MSLH-090）
