中国饲料Issue(1)：43-50,8.DOI:10.15906/j.cnki.cn11-2975/s.2024090002-12
海洋产尿酸氧化酶菌株的发酵优化及发酵放大
Fermentation optimization and fermentation scale-up of marine Urate oxidase-producing bacterial strains
摘要
Abstract
In order to improve the enzyme production capacity of Urate oxidase-producing strain Arthrobacter mysorens Dw-17a.Response surface methodology was used to optimize the enzyme-producing conditions for the fermentation of Dw-17a and fermentation scale-up experiments were carried out at a scale of 5 L.The results showed that the optimum fermentation conditions were temperature 15.6℃,pH 8.1,liquid filling amount 95 mL/500 mL,rotation speed 160 r/min and inoculum volume 3%.The average enzyme activity could reach 58.24 U/mL under this condition,which was 23.3%higher than that before optimization.On the basis of this experiment,a 5 L fermenter was scaled up,and the results showed that the optimal fermentation conditions for the 5 L scale were glucose 2%,yeast extract powder 1.5%,rotation speed 150 r/min,liquid filling amount 40%,and inoculum volume 3%,and the average enzyme activity could reach 42.11 U/mL under these conditions.关键词
迈索尔谷氨酸杆菌/尿酸氧化酶/响应面/发酵优化/发酵放大Key words
Arthrobacter mysorens/uric acid oxidase/response surface/fermentation optimization/fermentation scale-up分类
农业科技引用本文复制引用
王聪,迟雪梅,于爽,孙溪,郭信志,魏振勇,张庆芳,迟乃玉..海洋产尿酸氧化酶菌株的发酵优化及发酵放大[J].中国饲料,2026,(1):43-50,8.基金项目
国家重点研发项目(2022YFC2805105) （2022YFC2805105）
大连市科技创新基金项目(2020JJ26SN048) （2020JJ26SN048）
